Anti-RAS (K117N) Mouse Monoclonal Antibody-抗體-抗體-生物在線

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武漢紐斯特生物技術(shù)有限公司
Anti-RAS (K117N) Mouse Monoclonal Antibody

Anti-RAS (K117N) Mouse Monoclonal Antibody

商家詢價(jià)

產(chǎn)品名稱： Anti-RAS (K117N) Mouse Monoclonal Antibody

英文名稱： Anti-RAS (K117N) Mouse Monoclonal Antibody

產(chǎn)品編號(hào)： 26468

產(chǎn)品價(jià)格： null

產(chǎn)品產(chǎn)地： 美國(guó)

品牌商標(biāo)： NewEastbio

更新時(shí)間： null

使用范圍： ELISA, WB, IHC

武漢紐斯特生物技術(shù)有限公司
  • 聯(lián)系人 :
  • 地址 : 武漢市東湖新技術(shù)開(kāi)發(fā)區(qū)光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區(qū)域 : 湖北
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  • 郵箱 : neweast3@126.com

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Catalog Number:??26468
Gene Symbol:??H-Ras; K-Ras; N-Ras
Description: ??Anti-RAS (K117N) Mouse Monoclonal Antibody
Background: ??The K117N mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras K117N mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen: ??A synthetic peptide from the internal region of Ras which includes the mutation of K117N, human origin.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes K117N mutant, but not wild-type RAS of vertebrates.
Storage Conditions:?? Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant Ras (K117N) and wild-type proteins. Purified His-tagged Ras (K117N) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with anti-Ras (K117N) monoclonal antibody (Cat. #26468).
Immunofluorescence:??
Immunofluorescence of cells expressing KRas proteins with anti-KRas (K117N) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (WT) plasmid (left column) or pCDNA3-GFP-KRas (K117N) plasmid (right column), then fixed and stained with anti-KRas (K117N) monoclonal antibody (Cat. #26468).
Publications:
1.??DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
????Gene Therapy 21, 888-896 (October 2014)
2.??Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
????J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136