小鼠胰高血糖素即用型免疫組化試劑盒-分析方法-資訊-生物在線

?小鼠胰高血糖素即用型免疫組化試劑盒

英文名稱

Mouse Glucagon Ready-To-Use IHC Kit

中文名稱

小鼠胰高血糖素即用型免疫組化試劑盒

別????名

GCG; Glicentin related polypeptide; GLP 1; GLP 2; GLP1; GLP2; Glucagon like peptide 1; Glucagon like peptide 2; Glucagon precursor; GRPP; GLUC_HUMAN. ??

種????屬

Mouse

應用范圍

Immunohistochemistry

適用樣品基質

PFPE tissue

描述

IHC0104 is a ready-to-use IHC kit for staining of Glucagon. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

保存條件

Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

注意事項

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品介紹

產品組分及規格

編號	組分	規格	濃度	儲存
1	PBS緩沖液（干粉）	2 L×2	20x	室溫
2	抗原修復緩沖液	20 ml	100x	2-8℃
3	內源性過氧化物酶阻斷劑	3 ml	RTU	2-8℃
4	封閉工作液	3 ml	RTU	2-8℃
5	一抗（Mouse?Glucagon Rabbit?pAb）	6 ml	RTU	2-8℃
6	二抗（HRP-Goat anti-Rabbit IgG pAb）	6 ml	RTU	2-8℃
7	DAB kit（20×）顯色液	0.3 ml	RTU	-20℃
8	DAB kit（20×）稀釋液	0.3 ml	RTU	-20℃
9	復染試劑	5 ml	RTU	室溫
10	封片劑	5 ml	RTU	室溫
11	對照切片（小鼠胰腺）	1張	RTU	室溫
12	說明書	1份	?	?

背景

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.

石蠟包埋組織的免疫組織化學方案

1.?脫蠟水化

石蠟切片置于新鮮二甲苯中浸泡脫蠟3次，每次15 min；依次置于不同濃度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸餾水洗滌5 min，重復3次。

2.?抗原修復

沸水浴修復：將100×抗原修復緩沖液（試劑2）用蒸餾水稀釋成1×抗原修復緩沖液，放入修復盒中并提前加熱至95-100℃（注意蓋好以防液體蒸發），然后將切片放入修復盒中，在沸水浴環境中保持外沸狀態15 min，室溫自然冷卻；用PBS緩沖液（試劑1，將干粉溶解在2L蒸餾水中）清洗5 min，重復3次。

3.?阻斷內源性過氧化物酶

用吸水紙吸去玻片上多余的液體，用免疫組化筆在組織周圍畫圈，加入2-4滴內源性過氧化物酶阻斷劑（試劑3），室溫下置于濕盒中孵育15 min，用PBS洗滌5 min，重復3次。

4.?血清封閉

用吸水紙吸去玻片上多余的液體，加入2-4

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