肌動(dòng)蛋白α/α-SMA/α Actin單克隆即用型免疫組化試劑盒-分析方法-資訊-生物在線

?肌動(dòng)蛋白α/α-SMA/α Actin單克隆即用型免疫組化試劑盒

英文名稱

Alpha smooth muscle Actin Ready-To-Use IHC Kit

中文名稱

肌動(dòng)蛋白α/α-SMA/α Actin單克隆即用型免疫組化試劑盒

別????名

alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5??

種????屬

Human, Mouse, Rat

應(yīng)用范圍

Immunohistochemistry

適用樣品基質(zhì)

PFPE tissue

描述

IHC0114 is a ready-to-use IHC kit for staining of alpha smooth muscle Actin. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

保存條件

Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

注意事項(xiàng)

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產(chǎn)品介紹

產(chǎn)品組分及規(guī)格

編號(hào)	組分	規(guī)格	濃度	儲(chǔ)存
1	PBS緩沖液（干粉）	2 L×2	20x	室溫
2	抗原修復(fù)緩沖液	20 ml	100x	2-8℃
3	內(nèi)源性過(guò)氧化物酶阻斷劑	3 ml	RTU	2-8℃
4	封閉工作液	3 ml	RTU	2-8℃
5	一抗（Alpha smooth muscle Actin Mouse mAb）	6 ml	RTU	2-8℃
6	二抗（HRP-Goat anti-Mouse IgG pAb）	6 ml	RTU	2-8℃
7	DAB kit（20×）顯色液	0.3 ml	RTU	-20℃
8	? DAB kit（20×）稀釋液 ?	0.3 ml	RTU	-20℃
9	復(fù)染試劑	5 ml	RTU	室溫
10	封片劑	5 ml	RTU	室溫
11	對(duì)照切片（大鼠結(jié)腸，小鼠結(jié)腸，人子宮）	3張	RTU	室溫
12	說(shuō)明書(shū)	1份	?	?

背景

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

石蠟包埋組織的免疫組織化學(xué)方案

1.?脫蠟水化

石蠟切片置于新鮮二甲苯中浸泡脫蠟3次，每次15 min；依次置于不同濃度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸餾水洗滌5 min，重復(fù)3次。

2.?抗原修復(fù)

沸水浴修復(fù)：將100×抗原修復(fù)緩沖液（試劑2）用蒸餾水稀釋成1×抗原修復(fù)緩沖液，放入修復(fù)盒中并提前加熱至95-100℃（注意蓋好以防液體蒸發(fā)），然后將切片放入修復(fù)盒中，在沸水浴環(huán)境中保持外沸狀態(tài)15 min，室溫自然冷卻；用PBS緩沖液（試劑1，將干粉溶解在2L蒸餾水中）清洗5 min，重復(fù)3次。

3.?阻斷內(nèi)源性過(guò)氧化物酶

用吸水紙吸去玻片上多余的液體，用免疫組化筆在組織周圍畫(huà)圈，加入2-4滴內(nèi)源性過(guò)氧化物酶阻斷劑（試劑3），室溫下置于濕盒中孵育15 min，用PBS洗滌5 min，重復(fù)3次。

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