?一步法miRNA反轉錄試劑盒
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| 由于miRNA(microRNA)分子序列較小，僅20nt左右，沒有真核生物特有的Poly(A)尾巴結構，采用傳統Oligo dT引物和隨機引物很難獲得從miRNA到cDNA的反轉錄產物。目前普遍采用的解決方案有兩種：特異性反轉錄引物（圖1A所示）、兩步加A法反轉錄方案（圖1B所示）。 公司開發出一套全新的一步法miRNA反轉錄試劑盒，基于Peter改良的miR-Q技術，可以對成熟的miRNA同時完成Poly(A)反應和反轉錄反應，直接獲得含有特異性tag和15(T)的cDNA產物（如圖2所示）。該方法使得miRNA反轉錄與mRNA的反轉錄一樣輕松,直接將提取的microRNA與反應緩沖液混合物混合并置于PCR儀上1小時即可獲得高濃度的、包含所有miRNA分子的cDNA產物,獲得的cDNA產物適合于采用SYBR Green染料法進行RealTime PCR進行定量檢測。 |
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| 注：如4XOne step miRNA RT Solution出現沉淀屬正常現象，可用手捂化，混合均勻后使用不影響實驗結果。 | ||||||||||||
一步法microRNA反轉錄原理 |
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| 請置于-20°C，可保存2年；避免反復凍融。 | ||||||||||||
應用本產品文獻 |
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| 1.MicroRNA‐365 modulates astrocyte conversion into neuron in adult rat brain after stroke by targeting Pax6. 2.Grape Seed Procyanidin B2 Protects Porcine Ovarian Granulosa Cells against Oxidative Stress-Induced Apoptosis by Upregulating let-7a Expression. 3.Exosomes Derived From MicroRNA-148b-3p-Overexpressing Human Umbilical Cord Mesenchymal Stem Cells Restrain Breast Cancer Progression. 4.MicroRNA Expression Profiling in Newcastle Disease Virus-Infected DF-1 Cells by Deep Sequencing. 5.SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR‐25/Gsk3β‐mediated activation of Wnt/β‐catenin signaling. 6.LncRNA SNHG16 Functions as an Oncogene by Sponging MiR-135a and Promotes JAK2/STAT3 Signal Pathway in Gastric Cancer. 7. Circular RNA profiling identified an abundant circular RNA circTMTC1 that inhibits chicken skeletal muscle satellite cell differentiation by sponging miR-128-3p. 8.An innovative “unlocked mechanism” by a double key avenue for one-pot detection of microRNA-21 and microRNA-141. 9.Upregulation of miR-375 level ameliorates morphine analgesic tolerance in mouse dorsal root ganglia by inhibiting the JAK2/STAT3 pathway. 10.Transcriptome-Wide Identification and Characterization of MicroRNAs from Castor Bean (Ricinus communis L.). ? |
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