小鼠三葉肽因子3即用型免疫組化試劑盒-分析方法-資訊-生物在線

?小鼠三葉肽因子3即用型免疫組化試劑盒

英文名稱

Mouse TFF3 Ready-To-Use IHC Kit

中文名稱

小鼠三葉肽因子3即用型免疫組化試劑盒

別????名

Trefoil Factor 3; Trefoil factor3; hP1.B; Intestinal trefoil factor; ITF; mITF; OTTMUSP00000021729; Polypeptide P1.B; TFF3; TFI; TREFOIL; Trefoil factor (intestinal). ??

種????屬

Mouse

應用范圍

Immunohistochemistry

適用樣品基質

PFPE tissue

描述

IHC0103 is a ready-to-use IHC kit for staining of TFF3. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

保存條件

Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

注意事項

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品介紹

產品組分及規格

編號	組分	規格	濃度	儲存
1	PBS緩沖液（干粉）	2 L×2	20x	室溫
2	抗原修復緩沖液	20 ml	100x	2-8℃
3	內源性過氧化物酶阻斷劑	3 ml	RTU	2-8℃
4	封閉工作液	3 ml	RTU	2-8℃
5	一抗（Mouse?TFF3 Rabbit?pAb）	6 ml	RTU	2-8℃
6	二抗（HRP-Goat anti-Rabbit IgG pAb）	6 ml	RTU	2-8℃
7	DAB kit（20×）顯色液	0.3 ml	RTU	-20℃
8	? DAB kit（20×）稀釋液 ?	0.3 ml	RTU	-20℃
9	復染試劑	5 ml	RTU	室溫
10	封片劑	5 ml	RTU	室溫
11	對照切片（小鼠小腸）	1張	RTU	室溫
12	說明書	1份	?	?

背景

Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.

石蠟包埋組織的免疫組織化學方案

1.?脫蠟水化

石蠟切片置于新鮮二甲苯中浸泡脫蠟3次，每次15 min；依次置于不同濃度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸餾水洗滌5 min，重復3次。

2.?抗原修復

沸水浴修復：將100×抗原修復緩沖液（試劑2）用蒸餾水稀釋成1×抗原修復緩沖液，放入修復盒中并提前加熱至95-100℃（注意蓋好以防液體蒸發），然后將切片放入修復盒中，在沸水浴環境中保持外沸狀態15 min，室溫自然冷卻；用PBS緩沖液（試劑1，將干粉溶解在2L蒸餾水中）清洗5 min，重復3次。

3.?阻斷內源性過氧化物酶

用吸水紙吸去玻片上多余的液體，用免疫組化筆在組織周圍畫圈，加入2-4滴內源性過氧化物酶阻斷劑（試劑3），室溫下置于濕盒中孵育15 min，用PBS洗滌5 min，重復3次。

4.?血清封閉

用吸水紙吸去玻片上多余的液體，加入2-4滴封閉工作液（試劑4），置于濕盒內37℃封閉

上海信裕生物科技有限公司商家主頁

地址：上海市閔行莘莊工業區春東路508號A1-2F

聯系人：徐經理

電話： 021-60526354

傳真： 021-37680378

Email：shxysw02@163.com

女人与狗锁死了视频大全|霍金死亡过程恐怖视频|无节社团简谱季免费观看|六间房直播大厅|中文字幕欧美另类精品|久久人人玩人妻潮喷内射人人|亚洲国产一区二区三区在线观看