NMT是基因功能的活體檢測技術，已被103位諾貝爾獎得主所在單位，及北大、清華、中科院使用。
NMT歷史上的今天
2011年11月25日，山東省農科院董合忠、孔祥強利用NMT在Journal of Experimental Botany 上發表了標題為Effects of non-uniform root zone salinity on water use Na+ recirculation, and Na+ and H+ flux in cotton的研究成果。
期刊：Journal of Experimental Botany
主題：根系不均一鹽脅迫提升植物水分利用率的機制
標題：Effects of non-uniform root zone salinity on water use Na+ recirculation, and Na+ and H+ flux in cotton
影響因子：4.818
檢測指標：Na+、H+流速
作者：山東省農科院董合忠、孔祥強
英文摘要
A new split-root system was established through grafting to study cotton response to non-uniform salinity. Each root half was treated with either uniform (100/100?mM) or non-uniform NaCl concentrations (0/200 and 50/150?mM).
In contrast to uniform control, non-uniform salinity treatment improved plant growth and water use, with more water absorbed from the non- and low salinity side. Non-uniform treatments decreased Na+ concentrations in leaves. The [Na+] in the ‘0’ side roots of the 0/200 treatment was significantly higher than that in either side of the 0/0 control, but greatly decreased when the ‘0’ side phloem was girdled, suggesting that the increased [Na+] in the ‘0’ side roots was possibly due to transportation of foliar Na+ to roots through phloem.
Plants under non-uniform salinity extruded more Na+ from the root than those under uniform salinity. Root Na+ efflux in the low salinity side was greatly enhanced by the higher salinity side. NaCl-induced Na+ efflux and H+ influx were inhibited by amiloride and sodium orthovanadate, suggesting that root Na+ extrusion was probably due to active Na+/H+ antiport across the plasma membrane.
Improved plant growth under non-uniform salinity was thus attributed to increased water use, reduced leaf Na+ concentration, transport of excessive foliar Na+ to the low salinity side, and enhanced Na+ efflux from the low salinity root.
中文摘要（谷歌機翻）
通過嫁接建立了一個新的分根系統，以研究棉花對鹽度不均勻的響應。每個根部均用均一的（100/100 nonmM）或不均一的氯化鈉濃度（0/200和50/150 mM）處理。
與統一控制相反，非均勻鹽度處理改善了植物生長和水分利用，從非鹽度和低鹽度側吸收了更多的水。不均勻處理降低了葉片中的Na+濃度。0/200處理的'0'側根中的[Na+]顯著高于0/0對照中任一側的[Na+]，但當'0'側韌皮部被束腰時，其[Na+]大大降低，表明[ [0]側根中的Na+]可能是由于葉面Na+通過韌皮部轉運至根。
鹽度不均勻的植物比鹽度均勻的植物從根部擠出的Na+更多。低鹽度一側的根Na+流出量被高鹽度一側大大增強。NaCl誘導的Na+流出和H+流入被阿米洛利和原釩酸鈉抑制，這表明根Na+的擠出可能是由于跨質膜的活性Na+ / H+逆向轉運所致。
因此，在非均勻鹽度下植物生長的改善歸因于增加的水分利用，降低的葉片Na+濃度，過量的葉面Na+向低鹽度側的運輸以及從低鹽度根部提高的Na+外排。
Effects of non-uniform (0/200 mM and 50/150 mM NaCl) and uniform (0/0, 50/50, 100/100, and 150/150 mM NaCl) root zone salinity on net Na+ ?uxes in roots of cotton at 1 d (A) and 7 d (B) after treatment. The data are main ?uxes of Na+ within the measuring periods (15 min). Values are means 6SE (n?6). Bars with different letters (a, b, and c) differ signi?cantly at P < 0.05.
文章鏈接：
https://academic.oup.com/jxb/article/63/5/2105/531495?searchresult=1