NMT是基因功能的活體檢測技術，已被31位諾貝爾獎得主所在單位，及北大、清華、中科院使用。?
NMT歷史上的今天
2016年11月13日，中科院煙臺海岸帶研究所駱永明、李連禎利用NMT在Plant and Soil 上發(fā)表了標題為Determining the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique的研究成果。
期刊：Plant and Soil
主題：使用掃描離子選擇電極技術確定濕地植物根表面離子（Pb2+，Cu2+和Cd2+）的通量
標題：Determining the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique
影響因子：3.410
檢測指標：Pb2+、Cu2+、Cd2+流速
作者：中科院煙臺海岸帶研究所駱永明、李連禎
英文摘要
Measuring specific ion fluxes from different regions of the root under practical physiological conditions is crucial for understanding metal uptake mechanisms by plants.
We developed and tested a neutral carrier-based liquid-membrane Pb2+ and Cu2+ ion selective microelectrode (ISME) to investigate ion-transport processes along the roots of three common wetland plant species.
The Pb2+ and Cu2+ ISME exhibited a Nernstian response with Pb2+ and Cu2+ activities as low as 1.0 nM and 1.0 μM in deionized water and simulated soil solution, respectively. Phragmites australis had a region of Cu2+ release for approximately the first 200 μm, while it exhibited Pb2+ and Cd2+ outward net flux up to the first 500 μm. Although in older sections of the root of Phragmites australis there were areas of influx of Cu2+, Pb2+ and Cd2+, the overall influx was much smaller than that of Typha latifolia or Canna indica. Such a reduced uptake and/or an increased efflux of metal ions across the root-cell plasma-membrane might explain the higher resistance of Phragmites australis to metals, at least in part.
The Pb2+ and Cu2+ ISMEs are shown to permit detailed investigation of heavy-metal ion transport in plant roots, especially for plants used for phytoremediation.
中文摘要（谷歌機翻）
在實際的生理條件下，測量來自根不同區(qū)域的比離子通量對于理解植物對金屬的吸收機理至關重要。
我們開發(fā)并測試了基于中性載體的液膜Pb2+和Cu2+離子選擇性微電極（ISME），以研究沿三種常見濕地植物物種根部的離子遷移過程。
在去離子水和模擬土壤溶液中，Pb2+和Cu2+的ISME表現(xiàn)出Nernstian響應，Pb2+和Cu2+的活性分別低至1.0 nM和1.0μM。蘆葦在大約最初的200μm處具有Cu2+釋放區(qū)域，而在最初的500μm處表現(xiàn)出Pb2+和Cd2+向外凈通量。盡管在蘆葦根部的較老部分中有Cu2+，Pb2+和Cd2+的流入?yún)^(qū)域，但總流入量比香蒲或印度香豆小得多。穿過根細胞質(zhì)膜的這種減少的金屬離子吸收和/或增加的金屬離子外流可能至少部分解釋了蘆葦對金屬的更高抗性。
研究表明，Pb2+和Cu2+ ISME可以詳細研究植物根特別是用于植物修復的植物中重金屬離子的轉(zhuǎn)運。
Fig. 3 Measurement of fluxes (outward positive) of Cd2+,Pb2+ and Cu2+ (mean ± standard error) across the root tips of three common wetland plant species, Typha latifolia (a) Canna indic (b) and Phragmites australis (c), using Cd2+,Pb2+ and Cu2+ ion selective microelectrodes and the scanning ion-selective electrode higher metal influx in the root regions, especially in the meristematic and elongation zone (Fig. 3).
文章鏈接：
http://link.springer.com/10.1007/s11104-016-3109-5