NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺的必備技術(shù)。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學(xué)，正式打開歐洲市場。
研究使用平臺：NMT作物營養(yǎng)創(chuàng)新科研平臺
期刊：International Journal of Molecular Sciences
主題：赤霉素在調(diào)節(jié)玉米N吸收及其生理特性中的作用
標(biāo)題：The Role of Gibberellins in Regulation of Nitrogen Uptake and Physiological Traits in Maize Responding to Nitrogen Availability
影響因子：4.183
檢測指標(biāo)：NO3-流速
檢測樣品：玉米幼苗
NO3-流實(shí)驗(yàn)處理方法：
低氮/正常氮處理5天后檢測根系，檢測位置為根上距離根冠700微米的點(diǎn)。
NO3-流實(shí)驗(yàn)測試液成份：
0.1 mM NH4NO3、0.1mMKCl，0.1mMCaCl2、0.3mMMES，pH 6.0
作者：中國農(nóng)業(yè)大學(xué)張明才、王玉斌
中文摘要（谷歌機(jī)翻）
修飾的赤霉素（GA）信號導(dǎo)致作物中的低氮（N）使用效率（NUE）低的半侏儒癥。GA介導(dǎo)的N攝取的理解對于NUE改善的農(nóng)作物的發(fā)展至關(guān)重要。
在低（LN）和充足（SN）N條件下生長的GA合成缺陷型突變體zmga3ox中，分析了GA在調(diào)節(jié)氮吸收能力和**鹽（NO3-）轉(zhuǎn)運(yùn)蛋白（NRT）中的功能。LN顯著抑制了GA1，GA3和GA4的產(chǎn)生，而zmga3ox植物對芽和LN脅迫顯示出更高的敏感性。
此外，還記錄了較高的花青素積累和葉綠素含量的降低。在LN和SN條件下，zmga3ox植物的凈NO3-通量和15N含量均降低。外源GA3可以恢復(fù)zmga3ox植物的NO3-吸收，但烯康唑則抑制NO3-的吸收。
此外，ZmNRT2.1 / 2.2的轉(zhuǎn)錄水平在zmga3ox植物中被下調(diào)，而GA3的應(yīng)用提高了表達(dá)水平。此外，RNA-seq分析鑒定了一些轉(zhuǎn)錄因子，這些轉(zhuǎn)錄因子與GA介導(dǎo)的NRT相關(guān)基因的轉(zhuǎn)錄操作有關(guān)。
這些發(fā)現(xiàn)表明，GAs影響了NRTs的轉(zhuǎn)錄調(diào)控和玉米對氮素供應(yīng)的生理反應(yīng)中N的吸收。
Nitrateuptake and allocation in zmga3ox plants under LN and SN conditions. (a–b) The net NO3- flux (a), and the mean NO3- flux (b) along the maturation zone of wild-typeandzmga3ox primary root. Values werethe means ± SD (n =7). (c) The 15N content after 10 min15Ntracing assay in wildtype and zmga3ox plants; (d) total N content per plantin shoots and roots of wild-type and zmga3ox plants; (e) the 15N content in wild-type and zmga3oxseedlings treated with GA3 and/or uniconazole (Ucz). (c–e) Values were the means ±SD (n = 3). Different lettersindicated significant difference calculated by Fisher’s LSD (p < 0.05).
聯(lián)盟推薦抗疫產(chǎn)品之一：新冠肺炎干細(xì)胞治療NMT創(chuàng)新平臺
英文摘要
Modified gibberellin (GA) signaling leads to semi-dwarfism with low nitrogen (N) use effciency (NUE) in crops. Anunderstanding of GA mediatedNuptake is essential for the development of crops with improved NUE.
The function of GA in modulating N uptake capacity and nitrate (NO3-) transporters (NRTs) was analyzed in the GA synthesis-deficient mutant zmga3ox grown under low (LN) and suffcient (SN) N conditions. LN significantly suppressed the production of GA1, GA3, and GA4, and the zmga3ox plants showed more sensitivity in shoots as well as LN stress.
Moreover, the higher anthocyanin accumulation and the decrease of chlorophyll content were also recorded. The net NO3- fluxes and 15N content were decreased in zmga3ox plants under both LN and SN conditions. Exogenous GA3 could restore the NO3- uptake in zmga3ox plants, but uniconazole repressed NO3- uptake.
Moreover, the transcript levels of ZmNRT2.1/2.2 were downregulated in zmga3ox plants, while the GA3 application enhanced the expression level. Furthermore, the RNA-seq analyses identified several transcription factors that are involved in the GA-mediated transcriptional operation of NRTs related genes.
These findings revealed that GAs influenced N uptake involved in the transcriptional regulation of NRTs and physiological responses in maize responding to nitrogen supply.