葡聚糖硫酸鈉鹽（Dextran Sulfate Sodium Salt, DSS）是一種聚陰離子衍生物，其誘導結腸炎模型的機制雖仍未十分明確，但通常認為與巨噬細胞功能失調、腸道菌群失調、DSS對結腸上皮的毒性作用、細胞因子在DSS結腸炎模型的發病中起重要作用等機制有關。

實驗案例1

實驗動物：大鼠，1周齡，100 g；

實驗步驟：5%的DSS，3.5 mL/100 g每日灌胃，灌胃2周，HE染色；

實驗結果：UC大鼠的結腸長度低于健康大鼠；結腸黏膜異常，存在大量炎癥細胞。

圖1. (a)健康大鼠和UC大鼠的腸道長度;(b)結腸炎切片HE染色結果[1]

實驗案例2

實驗動物：大鼠，8周齡，200 g；

實驗步驟：飲用3.5% 濃度的DSS，連續飲用10天，HE染色；

實驗結果：結腸黏膜異常。

圖2. 健康大鼠和UC大鼠結腸炎切片HE染色結果[2]

實驗案例3

實驗動物：大鼠，7周齡，300-320 g；

實驗步驟：飲用7天 2.0 % 濃度的DSS后，飲用7天蒸餾水，共4個周期，HE染色；

實驗結果：結腸黏膜異常。

圖3. UC大鼠造模方法和結腸炎切片HE染色結果[3]

翌圣DSS產品優勢

翌圣生物提供高品質DSS（Cat.NO：60316ES，MW:36000~50000），具有以下優勢：

1. 高純度（＞98%），硫含量17-19%，游離硫＜0.2%，大量數據文獻支持，廣泛應用于UC模型的構建。

2. 多種屬動物中均可造模：小鼠、大鼠、斑馬魚、豬、果蠅；

3. 癥狀表現與人UC極度相似：可用于研究急、慢性結腸炎的發生發展機制，也可用于藥物的藥效研究。

4. 簡單易行，成模率高，重復性強

5. 聯合氧化偶氮甲烷 (azoxymethane, AOM)用藥，可用于誘發結腸炎相關性癌癥 (colitis associated cancer, CAC) 動物模型，成功模擬IBD誘發CAC的過程。 

建模效率與進口產品一致，產品價格低至進口價格的1/3，大量現貨庫存。

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近幾年客戶使用翌圣DSS已發表文獻（部分）

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2024

[1] Zhong D, Jin K, Wang R, ,et al. Microalgae-Based Hydrogel for Inflammatory Bowel Disease and Its Associated Anxiety and Depression. Adv Mater. 2024 Jan 26:e2312275. doi: 10.1002/adma.202312275. IF=29.4

[2] Xuan Zhang, Xia Gao, Xiangzhou Yi,et al. Multi-targeting inulin-based nanoparticles with cannabidiol for effective prevention of ulcerative colitis[J]. Materials Today Bio.2024, 25100965. IF=8.2

2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

2021

[1] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[2] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[3] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[4] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[5] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

參考文獻

[1] Zhou L, Yan F, Jiang R, et al. Administration of Nrf-2-Modified Hair-Follicle MSCs Ameliorates DSS-Induced Ulcerative Colitis in Rats[J]. Oxid Med Cell Longev.

[2] Li B, Du P, Du Y, et al. Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats[J]. Life Sci.

[3] Song G, Gan Q, Qi W, et al. Fructose Stimulated Colonic Arginine and Proline Metabolism Dysbiosis, Altered Microbiota and Aggravated Intestinal Barrier Dysfunction in DSS-Induced Colitis Rats. Nutrients.

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