Thrombin from bovine plasma 凝血酶

Synonym:

Factor IIa;Thrombin;凝血酶

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Product Description

Catalog Number:T4648

Storage Temperature: –20℃

CAS RN:9002-04-4

EC 3.4.21.5

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Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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The predominant form of thrombin in vivo is the zymogen, prothrombin (factor II), which is produced in the liver. The concentration of prothrombin in normal human plasma is 5–10 mg/dL.3 Prothrombin is a glycoprotein with a glycan content of ?12%.

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Prothrombin is cleaved in vivo by activated factor X, releasing the activation peptide and cleaving thrombin into light and heavy chains yielding catalytically active ?-thrombin. ?-Thrombin is composed of a light chain (A chain, MW ?6 kDA) and a heavy chain (B chain,

MW ?31 kDa). These two chains are joined by one disulfide bond.4 The B chain of human thrombin consists of a peptide portion (MW 29,485 Da) and a carbohydrate portion (MW 2,334 Da) with N-linked glycosylation at three Asn residues.5,6 Bovine thrombin contains 1.7% glucosamine, 1.8% sialic acid, 0.61% galactose, and 0.95% mannose. ?

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Thrombin also contains ?-carboxyglutamyl residues. These modified glutamyl residues are the result of carboxylation by a microsomal enzyme, vitamin Kdependent carboxylase. ?-Carboxyglutamyl residues are necessary for the Ca2+-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.4 Prothrombin is activated in vivo on the surface of a phospholipid membrane that binds the amino terminus of prothrombin along with factors Va and Xa. The activation process starts slowly because factor V is activated to factor Va by the initial, small amount of thrombin.

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Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

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Preparation Instructions

The product is soluble in water (10 mg/ml), yielding a clear solution.

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Storage/Stability

Stock solutions can be prepared at a concentration of 100 units/ml in a 0.1% (w/v) BSA solution. Stock solutions remain active for one week at 0–5℃. Solutions are most stable at pH 6.5, as a pH 7 will greatly reduce thrombin activity. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at –20℃ for long-term storage. Store the lyophilized powder at –20℃. The product retains activity for at least 6 years.

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Ordering Information

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