背景概述
腸道通透性增加和相關(guān)體重減輕是DSS結(jié)腸炎動(dòng)物模型的常?特征。D-甘露醇是?種惰性小碳水化合物分?，可沿整個(gè)隱窩-絨毛軸吸收，?于評(píng)估體內(nèi)腸道通透性。
圖源百度
實(shí)驗(yàn)動(dòng)物
4-5天大，約克郡小豬
實(shí)驗(yàn)材料
DSS（yeasen DSS 60316ES,MW36000-50000）
實(shí)驗(yàn)步驟
1. 以商業(yè)代乳粉配?飲?，每天喂食三次，直到實(shí)驗(yàn)結(jié)束；
2. 將動(dòng)物分為陽(yáng)性對(duì)照組（Pos），陰性對(duì)照組（Neg），實(shí)驗(yàn)組（Trp）;
3. 陽(yáng)性對(duì)照組（Pos）：灌注DSS;陰性對(duì)照組（Neg）：灌注生理鹽水;實(shí)驗(yàn)組（Trp）:灌注DSS+治療藥物；
4. 每只小豬每日攝入量1.25g DSS/kg，灌注5天。
實(shí)驗(yàn)結(jié)果
圖1. 腸道通透性分析
DSS誘導(dǎo)致小豬體內(nèi)D-甘露醇濃度升高，腸道屏障被破壞。
圖2. HE病理染色切片（A為Neg組；B為Pos組）
DSS處理的Pos組隱窩結(jié)構(gòu)扭曲、炎癥細(xì)胞浸潤(rùn)到粘膜和粘膜下層、隱窩膿腫和隱窩炎。
圖3. 炎癥因子檢測(cè)
DSS處理的Pos組中兩種細(xì)胞因?的濃度均升高[1]。
翌圣DSS產(chǎn)品優(yōu)勢(shì)
翌圣提供高品質(zhì)DSS（Cat.NO：60316ES，MW:36000~50000），具有以下優(yōu)勢(shì)：
1、高純度（＞98%），硫含量17-19%，游離硫＜0.2%，大量數(shù)據(jù)文獻(xiàn)支持，廣泛應(yīng)用于UC模型的構(gòu)建。
2、多種屬動(dòng)物中均可造模：小鼠、大鼠、斑馬魚、豬、果蠅；
3、癥狀表現(xiàn)與人UC極度相似：可用于研究急、慢性結(jié)腸炎的發(fā)生發(fā)展機(jī)制，也可用于藥物的藥效研究。
4、簡(jiǎn)單易行，成模率高，重復(fù)性強(qiáng)
5、聯(lián)合氧化偶氮甲烷 (azoxymethane, AOM)用藥，可用于誘發(fā)結(jié)腸炎相關(guān)性癌癥 (colitis associated cancer, CAC) 動(dòng)物模型，成功模擬IBD誘發(fā)CAC的過程。
建模效率與進(jìn)口產(chǎn)品一致，產(chǎn)品價(jià)格低至進(jìn)口價(jià)格的1/3，大量現(xiàn)貨庫(kù)存。
產(chǎn)品訂購(gòu)
|
產(chǎn)品名稱 |
貨號(hào) |
規(guī)格 |
價(jià)格（元） |
促銷價(jià)（元） |
|
60316ES25 |
25 g |
1255.00 |
955.00 |
|
|
60316ES60 |
100 g |
3855.00 |
2655.00 |
|
|
60316ES76 |
500 g |
16855.00 |
9655.00 |
|
|
60316ES80 |
1 kg |
32855.00 |
18655.00 |
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2022
[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143
[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451
[4] Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076
[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869
2021
[1] Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744
[2] Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
[3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717
[4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943
[5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
[6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
2020
[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561
[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
往期精彩
1. 不同分子量葡聚糖硫酸鈉選購(gòu)指南：高品質(zhì)DSS葡聚糖硫酸鈉鹽
2. 小鼠造模方法及具體步驟：Dextran Sulfate Sodium Salt（DSS）結(jié)腸炎造模解決方案 (yeasen.com)
3. DSS結(jié)腸炎造模整體解決方案：Dextran Sulfate Sodium Salt（DSS） 潰瘍性結(jié)腸炎模型的建立 (yeasen.com)
參考文獻(xiàn)
[1] Connie J. Kim, et al. Journal of Nutritional Biochemistry 21 (2010) 468–475.