JHM:NMT發現楊樹雌雄株吸鎘速率差異揭示其不同耐鎘策略-自主發布-資訊-生物在線

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JHM:NMT發現楊樹雌雄株吸鎘速率差異揭示其不同耐鎘策略

作者:旭月(北京)科技有限公司 2020-06-01T13:50 (訪問量:4866)

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NMT作為生命科學底層核心技術,是建立活體創新科研平臺的必備技術。2005~2020年,NMT已扎根中國152020年,中國NMT銷往瑞士蘇黎世大學,正式打開歐洲市場。

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研究使用平臺NMT植物重金屬創新科研平臺

期刊Journal of Hazardous Materials

主題NMT發現楊樹雌雄株根系吸Cd速率差異揭示其不同耐Cd策略

標題Are males and females of Populus cathayana differentially sensitive to Cd stress

影響因子7.65

檢測指標Cd2+流速

檢測樣品:楊樹

Cd2+流實驗處理方法

雌雄楊樹幼苗在050μM CdCl2中處理120

Cd2+流實驗測試液成份

0.05 mM CdSO40.25 mM NaCl0.05 mM KCl0.1 mM Na2SO4pH 6.0

作者:杭州師范大學李春陽、劉秒

中文摘要(谷歌機翻)

這項研究闡明了白楊在雄性和雌性中鎘吸收,轉運和排毒的機理,并揭示了一種雌雄異株的植物應對鎘污染的新策略。

雌性顯示出高水平的Cd吸收和從根到莖的移位,而雄性則顯示出根中大量Cd積累,提高的抗氧化能力以及有效的細胞和樹皮Cd螯合。

我們的研究還發現,鎘主要位于雄性根和葉的表皮和皮質組織中,而在雌性中,根和葉的維管組織以及葉肉中存在更多的鎘。另外,雄性中硫(S)和磷(P)的分布與鎘的分布非常相似,但雌性的相關性較弱。

掃描電子顯微鏡和能譜分析表明,雄性中組織鎘的含量與磷和磷含量呈正相關,而雌性中磷含量和硫含量卻沒有正相關(SCd之間的相關性較弱)。轉錄數據表明,Cd脅迫促進了雌性與Cd吸收和轉運相關的基因的上調,而雄性則促進了與細胞壁生物合成,金屬耐受性和次級代謝有關的基因的上調。

我們的研究結果表明,與雌性相比,對鎘脅迫的協調生理,微觀結構和轉錄應答賦予雄性更高的鎘耐受性,并且為對鎘脅迫的性差異應答的潛在機制提供了新見解。

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Net?Cd2+?fluxes in apical and mature zones of roots

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英文摘要

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This study clarifies the mechanisms of Cd uptake, translocation and detoxification in Populus cathayana Rehder females and males, and reveals a novel strategy for dioecious plants to cope with Cd contamination.

Females exhibited a high degree of Cd uptake and root-to-shoot translocation, while males showed extensive Cd accumulation in roots, elevated antioxidative capacity, and effective cellular and bark Cd sequestration.

Our study also found that Cd is largely located in epidermal and cortical tissues of male roots and leaves, while in females, more Cd was present in vascular tissues of roots and leaves, as well as in leaf mesophyll. In addition, the distributions of sulphur (S) and phosphorus (P) were very similar as that of Cd in males, but the associations were weak in females.

Scanning electron microscopy and energy spectroscopy analyses suggested that the amounts of tissue Cd were positively correlated with P and S amounts in males, but not in females (a weak correlation between S and Cd). Transcriptional data suggested that Cd stress promoted the upregulation of genes related to Cd uptake and translocation in females, and that of genes related to cell wall biosynthesis, metal tolerance and secondary metabolism in males.

Our results indicated that coordinated physiological, microstructural and transcriptional responses to Cd stress endowed superior Cd tolerance in males compared with females, and provided new insights into mechanisms underlying sexually differential responses to Cd stress.

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關鍵詞:非損傷微測技術Cd2+速,楊樹,植物重金屬