GelRed

歡迎致電北京方程生物 010-57266903

Biotium公司的GelRed 和 GelGreen 無論用于預制凝膠染色還是凝膠電泳后染色，都表現出了極高的靈敏度。為配合 312 nm UV 凝膠成像系統而設計的 GelRed ，在使用了我們新開發的具有**的試驗步驟后（該試驗步驟中使用稀釋的 NaCl 溶液替代傳統的 TBE 緩沖溶液），在凝膠電泳后染色中優于或者至少相當于 SYBR Gold 。但與 SYBR Gold 不同， GelRed 在預制凝膠中使用時仍然有極高的靈敏度，事實上GelRed 在檢測低濃度、微量 DNA 方面比 EB 表現出更高的靈敏度，尤其對小分子量的 DNA 檢測非常靈敏（圖1）。 GelGreen 可以滿足使用 488 nm 激光凝膠掃描儀或者可見光激發的 Dark Reader 的研究人員的使用要求。 GelGreen 無論是在預制凝膠還是凝膠電泳后染色中都與 SYBR Green I 靈敏度相當，但不存在后者的不穩定性問題。實際上， GelRed 和 GelGreen ，特別是 GelRed 非常穩定，可以長期在室溫下保存。當這兩種染料稀釋在 TBE 或者類似的電泳緩沖溶液中時甚至可以使用微波爐加熱，便于采用常規方法制備預制凝膠。含有染料的預制凝膠可以成批制備，長期保存。GelRed

Product Description

GelRed? is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed? is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed? and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed? without changing your existing imaging system.

GelRed? can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.

A series of safety tests have confirmed that GelRed? is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed? can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download the GelRed/GelGreen Safety Report.

For new users, Biotium recommends GelRed? 10,000X solution in water (41003), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelRed? 10,000X solution in DMSO for established users of GelRed? in DMSO who do not wish to change their laboratory protocols. The performance and stability of GelRed? 10,000X is comparable in both the water and DMSO formulations. For your convenience, we also offer ready-to-use GelRed? 3X in water (41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10 mL.

FEATURES

Safer than EB: Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
Easy disposal: Passed environmental safety tests for direct disposal down the drain or in regular trash
Ultra-sensitive: Much more sensitive than EtBr and SYBR Safe
Extremely stable: Available in water, stable at room temperature for long-term storage and microwavable
Simple to use: Very simple procedures for either precast and post gel staining
Perfectly compatible with a standard UV transilluminator : GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)
Perfectly compatible with downstream applications : Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.
The Most Sensitive and Stable Precast Gel StainFigure 1.GelRed? is significantly more sensitive than ethidium bromide (EB) for detecting low-level DNA, especially in the lower molecular weight area. Shown left are two-fold serial dilutions of 1 Kb Plus DNA Ladder from Invitrogen electrophoresed on 1% agarose gels precasted with GelRed or EB in 1x TBE. The total amount of DNA loaded per lane was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300-nm transillumination and photographed with an EB filter and Polaroid 667 black-and-white print films.
The Most Sensitive and Stable Post Gel StainFigure 2. GelRed? displays consistently superior sensitivity for post gel staining, regardless of the filter used (A vs. C) and storage and handling condition. SYBR Gold, however, showed comparable performance only when used fresh from the manufacturer and with a SYBR filter (B vs. D). Following a few freeze-thaw cycles, SYBR Gold 10,000X solution degraded significantly, resulting in poor staining (E). SYBR Gold 1X solution also degrades over time (see Figure 4). Two-fold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were electrophoresed on 1% agarose gels in 1x TBE and post- stained with GelRed?and SYBR Gold, respectively. Gels were imaged using 300-nm transillumination and photographed with the indicated filters and Polaroid black-and-white print films. The total amount of DNA per lane for each serial dilution was: 200 ng, 100 ng, 50 ng and 25 ng from left to right.

同樣重要的是， GelRed 和 GelGreen 相比 EB 或 SYBR Green I 提高了安全性。 獨立的測試服務公司（ Litron Laboratories, Inc. ）進行的標準艾姆斯氏測試結果表明， 在 18.5 μ g /mL （該濃度遠高于推薦用于電泳后染色的 約 4 μ g/mL 的 3X 染色液 ）濃度下， GelGreen 沒有誘變性，而 GelRed 也僅在 S9 代謝活化時有微弱的誘變性。 GelRed 和 GelGreen 的特殊化學結構使其難以穿透細胞膜進入細胞，正是這一特性降低了染料的細胞毒性。如圖3。相反， SYBR Green I 廣泛地用于活細胞線粒體和核 DNA 染色，這正是由于它能快速的被細胞吞入。由于已知 SYBR Green I 對紫外線導致的突變有強烈的增強作用（ Ohta et al. Mutat. Res. 492 , 91(2001) ），因此它的高細胞膜透性使它在紫外環境觀察時成為凝膠染色的主要有害物質。GelRed