NMT歷史上的今天
2017年11月07日，首都師范大學印莉萍、譚松利用NMT在Traffic上發表了標題為TMD1 domain and CRAC motif determine the association and disassociation of MxIRT1 with detergent‐resistant membranes
的研究成果。
期刊：Traffic
主題：TMD1域和CRAC基序決定MxIRT1與耐去污劑膜的締合和解離
標題：TMD1 domain and CRAC motif determine the association and disassociation of MxIRT1 with detergent‐resistant membranes
影響因子：4.133
檢測指標：Cd2+流速
作者：首都師范大學印莉萍、譚松
英文摘要
Iron is essential for most living organisms. The iron‐regulated transporter1 (IRT1) plays a major role in iron uptake in roots, and its trafficking from endoplasmic reticulum (ER) to plasma membrane (PM) is tightly coordinated with changes in iron environment.
However, studies on the IRT1 response are limited. Here, we report that Malus xiaojinesis IRT1 (MxIRT1) associates with detergent‐resistant membranes (DRMs, a biochemical counterpart of PM microdomains), whereas the PM microdomains are known platforms for signal transduction in the PM. Depending on the shift of MxIRT1 from microdomains to homogeneous regions in PM, MxIRT1‐mediated iron absorption is activated by the cholesterol recognition/interaction amino acid consensus (CRAC) motif of MxIRT1.
MxIRT1 initially associates with DRMs in ER via its transmembrane domain 1 (TMD1), and thus begins DRMs‐dependent intracellular trafficking. Subsequently, MxIRT1 is sequestered in COPII vesicles via the ER export signal sequence in MxIRT1.
These studies suggest that iron homeostasis is influenced by the CRAC motif and TMD1 domain due to their determination of MxIRT1‐DRMs association.
中文摘要（谷歌機翻）
鐵對大多數生物都是必不可少的。鐵調節轉運蛋白1（IRT1）在根部吸收鐵中起主要作用，其從內質網（ER）到質膜（PM）的運輸與鐵環境的變化緊密相關。
但是，有關IRT1反應的研究有限。在這里，我們報告說，小金海棠IRT1（MxIRT1）與耐去污劑的膜（DRM，PM微域的生化對應物）相關，而PM微域是PM中信號轉導的已知平臺。取決于MxIRT1從PM的微區到均質區的轉移，MxIRT1介導的鐵吸收被MxIRT1的膽固醇識別/相互作用氨基酸共有（CRAC）基序激活。
MxIRT1最初通過其跨膜結構域1（TMD1）與ER中的DRM結合，從而開始依賴DRM的細胞內運輸。隨后，通過MxIRT1中的ER出口信號序列將MxIRT1隔離在COPII囊泡中。
這些研究表明，由于鐵的動態平衡決定了MxIRT1-DRM的相關性，因此受CRAC基序和TMD1域的影響。
G) Average Cd2+flux in the different transgenic Arabidopsis root hairs in 7-minute incubation with 0.05 mM CdCl2 measured by Non-invasion Micro-test Technology. The bars represent the means standard errors of 3 independent analyses (n = 3). Significant differences were determined by
Student’s t test. *P < 0.05, **P < 0.01
文章鏈接： https://linkinghub.elsevier.com/retrieve/pii/S0167880918304377