女人与狗锁死了视频大全|霍金死亡过程恐怖视频|无节社团简谱季免费观看|六间房直播大厅|中文字幕欧美另类精品|久久人人玩人妻潮喷内射人人|亚洲国产一区二区三区在线观看

不同的根區在胡楊屬物種中具有不同的吸收**鹽（NO₃^-）的能力，但基本的生理和微RNA（miRNA）調節機制仍然未知。

?

為了解決這個問題，研究了兩個具有不同NO3吸收能力的根莖假單胞菌的根區。到根尖的0至40 mm區域（根部區域I）顯示出凈流入，而40至80 mm的區域（根部區域II）顯示出凈流出。II區的NO₃^-和銨（NH₄⁺）濃度以及**還原酶（NR）活性低于I區。

?

與區域I相比，在區域II中鑒定了41個上調的miRNA和23個下調的miRNA，以及這些miRNA的576個靶標。尤其是生長調節因子4（GRF4），即ptc-miR396g-5p和ptc-miR396f_L上調的靶標與區域I相比，區域II中的+ 1R-1被下調，可能有助于區域II中較低的NO3吸收率和吸收。

?

此外，在根區發現了一些miRNA及其靶標，即C2H2鋅指家族成員和APETALA2 /乙烯反應性元素結合蛋白家族成員，這可能在調節NO₃^-吸收中起重要作用。這些結果表明，差異表達的miRNA-靶標對在調節不同NO₃^-吸收速率以及在楊樹不同根部區域的同化中起關鍵作用。

?

Different root zones have distinct capacities for nitrate (NO₃^-) uptake in Populus species, but the underlying physiological and microRNA (miRNA) regulatory mechanisms remain largely unknown.

?

To address this question, two root zones of P. × canescens with contrasting capacities forNO₃^- uptake were investigated. The region of 0 to 40 mm (root zone I) to the root apex displayed net influxes, whereas the region of 40 to 80 mm (root zone II) exhibited net effluxes. Concentrations of NO₃^- and ammonium (NH₄⁺) as well as nitrate reductase (NR) activity were lower in zone II than in zone I.

?

Forty one upregulated and twenty three downregulated miRNAs, and 576 targets of these miRNAs were identified in zone II in comparison with zone I. Particularly, growth-regulating factor 4 (GRF4), a target of upregulated ptc-miR396g-5p and ptc-miR396f_L + 1R-1, was downregulated in zone II in comparison with zone I, probably contributing to lower NO₃^-uptake rates and assimilation in zone II.

?

Furthermore, several miRNAs and their targets, members of C2H2 zinc finger family and APETALA2/ethylene-responsive element binding protein family, were found in root zones, which probably play important roles in regulating NO₃^- uptake. These results indicate that differentially expressed miRNA-target pairs play key roles in regulation of distinct NO₃^-uptake rates and assimilation in different root zones of poplars.