NMT作為生命科學底層核心技術，是建立活體創新科研平臺的必備技術。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學，正式打開歐洲市場。
研究使用平臺：NMT重金屬創新科研平臺
期刊：Environmental Pollution
主題：硅藻的[Si依賴性Cd/ Cu/ Pb耐受力]的機制
標題：Mechanisms underlying silicon-dependent metal tolerance in the marine diatom Phaeodactylum tricornutum
影響因子：5.714
檢測指標：Cd2+, Cu2+, Pb2+流速
檢測樣品：硅藻
Cd2+, Cu2+, Pb2+流實驗處理方法：
硅藻細胞在低硅（46μM）和高硅（260μM）中生長
Cd2+, Cu2+, Pb2+流實驗測試液成份：
10μM CdCl2，0.1 mM KCl, 0.1 mM MgCl2, 0.5 mM NaCl,0.3 mM MES, 0.2 mM Na2SO4, and 0.1% 蔗糖， pH 8.0
作者：深圳大學潘科、周貝貝
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中文摘要（谷歌機翻）
人為活動顯著改變了沿海水域的化學計量和養分濃度。由于氮，磷和硅酸鹽的投入不成比例，硅已成為潛在的限制養分。
營養物質比率的破壞會導致海洋硅藻中金屬敏感性的變化和積累，海洋硅藻是需要硅才能生長的重要的一組真核浮游植物。
在這項研究中，我們檢查了硅可用性對硅藻P. tricornutum中金屬敏感性的影響。我們發現硅饑餓嚴重損害了其鎘，銅和鉛的耐受性。有趣的是，多條證據表明，富硅細胞比缺硅細胞具有更高的金屬吸附和流入速率。
然而，富含Si的細胞還具有更高的響應能力，可以通過膜和液泡金屬轉運蛋白的表達增加以及更大的抗氧化活性來抵抗金屬毒性，這些抗氧化活性可以清除金屬應力產生的活性氧。
Fig. 3. Metal fluxes around the cell surface of individual P. tricornutum diatom. (A–C) Instantaneous net metal flux as measured by a non-invasive microelectrode probe. H-Si, high Si growth conditions; L-Si, low Si growth conditions; (D) the calculated mean net metal flux for the L-Si and H-Si cells. Asterisks indicate the L-Si and H-Si treatments significantly differ (***p ≤ 0.001).
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英文摘要
Anthropogenic activities have significantly changed the stoichiometry and concentrations of nutrients in coastal waters.Silicon (Si) has become a potential limiting nutrient due to disproportionate nitrogen, phosphorus, and silicate inputs into these areas.
The disrupted nutrient ratios can cause changes to metal sensitivity and accumulation in marine diatoms, an important group of eukaryotic phytoplankton that requires silicon for growth.
In this study, we examined the effects of Si availability on the metal sensitivity in the diatom P. tricornutum. We found that Si starvation dramatically compromised its cadmium, copper, and lead tolerances. Interestingly, multiple lines of evidence indicated that Si-enriched cells had higher metal adsorption and influx rates than Si-starved cells.
Yet Si-enriched cells also had a greater ability to respond to and counteract metal toxicity via elevated expression of membrane and vacuolar metal transporters and greater antioxidant activities which scavenge reactive oxygen species created by metal stress.