NMT歷史上的今天
2017年10月11日，魯東大學資源與環境工程學院傅金民利用NMT在Plant and soil上發表了標題為Application of Aspergillus aculeatus to rice roots reduces Cd concentration in grain的研究成果。
期刊：Plant and soil
主題：在水稻根部施用刺曲霉可降低谷物中的Cd濃度
標題：Application of Aspergillus aculeatus to rice roots reduces Cd concentration in grain
影響因子：3.306
檢測指標：Cd2+流速
檢測部位：水稻根部（距離根尖500μm）
Cd2+流實驗處理方法：
三周齡的水稻幼苗，50uM鎘處理、50uM鎘+ A. aculeatus處理7/14天
Cd2+流實驗測試液成份：
50 μMCdCl2, 0.5 mMKCl, 0.1 mM CaCl2, 0.1mMNaCl, 0.1mMMgCl2 and 0.3mM2-(Nmorpholino)ethane sulfonic acid (MES)
作者：魯東大學資源與環境工程學院傅金民
英文摘要
‘Cd toxicity in rice’ events have resulted in vast public concern and uncertainty. Effective bioremediation could be accomplished via applying microbes that are capable of alleviating Cd content in rice grains.
Here, we investigated the effect of inoculating Aspergillus aculeatus on tolerance, uptake and transportation of Cd in rice cultivated in Cd contaminated growth medium.
A. aculeatus facilitated rice growth in Cd polluted growth medium and alleviated Cd toxic effects according to our observations on biomass, leaf and root length and grain yield. Cd accumulation analysis indicated that the plants which were inoculated with A. aculeatus exhibited minimum Cd level in all organs. Particularly in grain we observed a 40.5% reduction compared to the Cd only treated plants. Differences in Cd accumulation in rice inoculated with A. aculeatus might be attributed to the enhancement of cell wall-bound Cd, decreasing the Cd inorganic forms in roots, and inhibiting the expression of OsNRAMP5 and OsNRAMP1. A. aculeatus inoculation also led to minimum growth medium DTPA-Cd concentration, which possibly reduced the availability of the metals for plant uptake.
These results suggested that A. aculeatus might potentially be applicable to improve Cd tolerance and reduce Cd transportation in grains of rice.
中文摘要（谷歌機翻）
大米事件中的鎘毒性已引起公眾的廣泛關注和不確定性。通過應用能夠減輕稻米中Cd含量的微生物，可以實現有效的生物修復。
在這里，我們研究了接種刺曲霉對Cd污染的生長培養基中種植的水稻對Cd的耐受性，吸收和運輸的影響。
根據我們對生物量，葉片和根長以及籽粒產量的觀察，A。aculeatus促進了水稻在Cd污染的生長培養基中的生長，并減輕了Cd的毒性作用。鎘的積累分析表明，接種了棘孢曲霉的植物在所有器官中都表現出最低的鎘水平。與僅使用鎘處理的植物相比，特別是在谷物中我們觀察到減少了40.5％。接種A. aculeatus的水稻中Cd積累的差異可能歸因于細胞壁結合的Cd的增強，根部中Cd無機形式的減少以及抑制OsNRAMP5和OsNRAMP1的表達。刺果曲霉的接種還導致生長培養基中DTPA-Cd的濃度降至最低，這可能會降低植物吸收金屬的有效性。
這些結果表明，刺果曲霉具有提高Cd耐性和減少Cd在水稻籽粒中運輸的潛力。
Supplementary Fig. 1 Effects of the Cd- resistant strain A. aculeatus on the net fluxes (a, c) and the mean fluxes (b, d) of Cd2+ in rice roots. Values are means ± SD. The different letters indicate the values that were significantly different at P < 0.05.
文章鏈接：https://link.springer.com/article/10.1007%2Fs11104-017-3465-9