轉自“中關村NMT聯盟”
NMT作為生命科學底層核心技術，是建立活體創新科研平臺的必備技術。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學，正式打開歐洲市場。
研究使用平臺：NMT煙草品質創新科研平臺
期刊：Journal of Nanobiotechnology
主題：碳納米顆粒促煙草鉀吸收機制
標題：Carbon nanoparticles enhance potassium uptake via upregulating potassium channel expression and imitating biological ion channels in BY?2 cells
影響因子：5.345
檢測指標：K+流速
檢測樣品：煙草
NO3-流實驗處理方法：
煙草BY-2懸浮細胞，分別在62.50μg/mL CNP，125.00μg/mL CNP和125.00μg/mL CNP+10mM TEA環境下處理12小時
NO3-流實驗測試液成份：
46.88 mM KCl, 0.1 mM CaCl2, 0.3 mM MES, pH 5.9
作者：中國煙草總公司鄭州煙草研究院謝劍平、陳麗娟
中文摘要（谷歌機翻）
碳納米顆粒（CNPs）可以促進植物生長，而到目前為止，尚未報道CNPs促進植物生長吸收鉀的機制。
在這項研究中，建立了CNPs促進BY-2細胞吸收鉀的功能，并且細胞中積累的鉀與細胞的新鮮生物量具有顯著相關性。細胞中的K+隨著CNP濃度的增加而增加。用CNP處理后，K+流入量達到高水平，并且顯著高于用K+阻斷劑氯化四乙銨（TEA+）處理的對照組和陰性組。
在CNPs抑制劑存在下，K+積累沒有減少。與對照組相比，在鉀通道阻滯劑TEA+或CNPs抑制劑的存在下，NKT1基因表達發生了變化。發現CNP比圓錐形納米毛細管中通過離子電流測定法（RIC）整流確定的其他陽離子優先運輸K+。
這些結果表明CNPs上調鉀基因表達以增強BY-2細胞中的K+。此外，據推測，CNP通過大量的羧基模擬了離子通道的蛋白質，以滲透K+。這些發現將為通過碳納米顆粒改善植物生長提供支持。
Effects of CNPs and inhibitor treatments on net K+ fluxes in BY-2 cells. a K fluxes were determined with after different CNPs treatments lasting for 900 s measurements. b Schematic diagram of test sample. c Mean value of K+ fluxes at different concentration of CNPs in cells. Group 1: 62.50 μg/mL CNPs, Group 2: 125.00 μg/mL CNPs, Group 3: 125.00 μg/mL CNPs + 10 mM TEA+. The mean value of each group contains six individual cell, and error bars represent SE, n = 6. Asterisk (*) indicates significant difference compared with control group (p < 0.05)
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英文摘要
In this study, the function that CNPs promoted potassium uptake in BY-2 cells was established and the potassium accumulated in cells had a significant correlation with the fresh biomass of cells. The K+ in cells increased with the increasing concentration of CNPs. The K+ influx reached high level after treatment with CNPs and was significantly higher than that of the control group and the negative group treated with K+ blocker, tetraethylammonium chloride ( TEA+).
The K+ accumulation was not reduced in the presence of CNPs inhibitors. In the presence of potassium channel blocker TEA+ or CNPs inhibitors, the NKT1 gene expression was changed compared with the control group. The CNPs were found to preferentially transport K+ than other cations determined by rectification of ion current assay (RIC) in a conical nanocapillary.
These results indicated that CNPs upregulated potassium gene expression to enhance K+ in BY-2 cells. Moreover, it was speculated that the CNPs simulated protein of ion channels via bulk of carboxyl for K+ permeating. These findings will provide support for improving plant growth by carbon nanoparticles.