RNA核酸外切酶2同源物(REXO2)重組蛋白說明書-分析方法-資訊-生物在線

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RNA核酸外切酶2同源物(REXO2)重組蛋白說明書

作者:上海滬震實業有限公司 2023-04-25T00:00 (訪問量:4207)

?RNA核酸外切酶2同源物(REXO2)重組蛋白說明書

Recombinant RNA Exonuclease 2 Homolog (REXO2)?

[ PROPERTIES ]

Residues: Gln41~Asn213

Tags: N-terminal His-Tag

Accession: Q9Y3B8

Host: E. coli

Subcellular Location: Mitochondrion.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 0.01% sarcosyl and 5%

trehalose.

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 21.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[USAGE]

Reconstitute in sterile ddH2O

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC??for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

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[ SEQUENCES ]

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The sequence of the target protein is listed below.

QRMVWVDLEM TGLDIEKDQI IEMACLITDS DLNILAEGPN LIIKQPDELL DSMSDWCKEHHGKSGLTKAV KESTITLQQA EYEFLSFVRQ QTPPGLCPLA GNSVHEDKKF LDKYMPQFMKHLHYRIIDVS TVKELCRRWY PEEYEFAPKK AASHRALDDI SESIKELQFY RNN

[ REFERENCES ]

1. Lai C.-H., et al. (2000) Genome Res. 10:703-713.

2. Nguyen L.H., et al. (2000) J. Biol. Chem. 275:25900-25906.

3. Mechold U., et al. (2006) Nucleic Acids Res. 34:2364-2373.

4. Van Damme P., et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454.

2022121705182335588

本產品僅供科研使用。不能用于人和動物治療等其它臨床診斷使用

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