本試劑盒采用夾心ELISA技術，可特異性識別人和小鼠 α-突觸核蛋白寡聚體，與α-突觸核蛋白單體和原纖維的交叉反應性極低(參見圖1結果)，確保檢測結果真實反映寡聚體水平，有效避免非特異性結合導致的數據偏差。

圖1. The graph demonstrates robust detection of Human Recombinant Alpha Synuclein Oligomers (Cat#SPR-484) and limited cross-reactivity with Human Recombinant Alpha Synuclein Pre-Formed Fibrils and Monomers (Cat #s SPR-322 & SPR-321).

試劑盒已針對腦組織勻漿樣本進行了全面驗證，并在說明書中提供了詳細的樣品制備方案，幫助您高效處理腦組織樣本。在轉基因動物模型研究中，可對腦組織勻漿中α-突觸核蛋白寡聚體實現穩定、可靠的定量分析(參見圖2結果)。

圖2. Representative quantification of mouse brain homogenate samples of unknown concentrations of Alpha Synuclein Oligomers against a standard curve of Human Recombinant Alpha Synuclein Oligomers when using StressMarq’s Alpha Synuclein Oligomer ELISA kit (Cat # SKT-143). A: Non-detergent protein extraction from brain homogenates of hemizygous M83 mice injected in the striatum with either Human Recombinant Alpha Synuclein Pre-Formed Fibrils (PFF; n = 3) or with buffer (PBS; n = 3). B: Non-detergent protein extraction from brain homogenates of either homozygous M83 mice (M83 Transgenic; n = 2) or wildtype mice (Wildtype;n = 2). C-D: Detergent-based protein extraction (25 ug/mL) from brain homogenates of hemizygous M83 mice injected in the striatum with either PFF or PBS. Data from males (C: PFF n = 6; PBS n = 6) and females (D: PFF n = 6; PBS n = 5) are presented separately. Experimental data courtesy of Rodrigo Sandoval Contreras and Dareen Abdel-Rahman from the Prado Lab at University of Western Ontario, contributed as part of a collaborative research project.

試劑盒檢測靈敏度達10pg/mL，檢測范圍為15.6-1000pg/mL，可覆蓋生理到病理狀態下的寡聚體濃度變化。標準曲線線性良好(參見圖3結果)，為精確定量提供可靠保障。

圖3. Typical Standard Curve for the Alpha Synuclein Oligomer ELISA Kit (SKT-143). Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 15.6–1,000 pg/ml.

試劑盒包含所有必需組分，并配套詳細操作說明書。從樣品孵育到顯色讀數，整個流程僅需約3小時，支持高通量檢測，可同時檢測40個樣本(含復孔)。