碘甲腺原氨酸脫碘酶Ⅰ(DIO1)重組蛋白說明書
Recombinant Deiodinase, Iodothyronine, Type I (DIO1)?
[ DESCRIPTION ]
?Protein Names:?Deiodinase, Iodothyronine, Type I
?Synonyms:DIO1, Itdi1
Species: Rat
Size: 100μg
Source:Escherichia coli-derived
Subcellular Location?:Endoplasmic reticulum membrane; Single-pass membraneprotein.
[ PROPERTIES ]
Residues:?Thr31~Phe257 with N-terminal His-Tag
Grade & Purity:?>95%, 27 kDa as determined by SDS-PAGE reducing conditions.
?Formulation:?Supplied as lyophilized form in PBS, pH 7.4, containing 0.01% Sarcosyl, 5% sucrose.
?Endotoxin Level:?<1.0 EU per 1μg (determined by the LAL method).
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Molecular Mass:?27.80kDa
Predicted isoelectric point: 8.9
[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
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Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37oC?for 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.
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[ SEQUENCES]
The target protein is fused with N-terminal His-tag, its sequence is listed below.
MGHHHHHHSG SEF-TLFPERV KQNILAMGQK TGMTRNPRFA PDNWVPTFFS IQYFWFVLKV RWQRLEDRAE YGGLAPNCTVVRLSGQKCNV WDFIQGSRPL VLNFGSCTUP SFLLKFDQFK RLVDDFASTA DFLIIYIEEA HATDGWAFKN NVDIRQHRSLQDRLRAAHLL LARSPQCPVV VDTMQNQSSQ LYAALPERLY VIQEGRICYK GKPGPWNYNP EEVRAVLEKL CIPPGHMPQF
[ REFERENCES ]
1. Berry M.J., et al. (1991) Nature 349:438-440.
2. The MGC Project Team. (2004) Genome Res. 14:2121-2127.
3. Berry M.J., (1992) J. Biol. Chem. 267:18055-18059.
4. Macejova D., et al. (2001) Gen. Physiol. Biophys. 20:293-302.
本產品僅供科研使用。不能用于人和動物治療等其它臨床診斷使用