?17-β-羥基類固醇脫氫酶3(HSD17b3)重組蛋白說明書
Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)?
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Met1~Arg310
Tags: N-terminal His Tag
Subcellular Location: Endoplasmic reticulum lumen
Purity: > 90%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.6, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 120μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 38.2kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
本產(chǎn)品僅供科研使用。不能用于人和動物治療等其它臨床診斷使用