鞘磷脂蛋白0(MPZ)重組蛋白說明書
Recombinant Protein Zero, Myelin (MPZ)?
[PROPERTIES]
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Residues:? Ile30~Lys248with N-terminal His-Tag.
Host: E. coli
Subcellular? Location: Cell membrane;Single-pass type I membrane protein.
Purity: >95%
Endotoxin? Level: <1.0EU per 1μg(determined by the LAL method).
Formulation:? Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted? isoelectric point: 9.5
Predicted Molecular Mass: 26.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[USAGE]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC?for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition
[ SEQUENCES ]?
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The target protein is fused with N-terminal His-Tag, its sequence is listed below.?
MGHHHHHHSG SEF- I VVYTDREVHG AVGSRVTLHC SFWSSEWVSD DISFTWRYQPEGGRDAISIF HYAKGQPYID EVGTFKERIQ WVGDPRWKDG SIVIHNLDYS DNGTFTCDVKNPPDIVGKTS QVTLYVFEKV PTRYGVVLGA VIGGVLGVVL LLLLLFYVVR YCWLRRQAALQRRLSAMEKG KLHKPGKDAS KRGRQTPVLY AMLDHSRSTK AVSEKKAKGL GESRKDKK
本產(chǎn)品僅供科研使用。不能用于人和動物治療等其它臨床診斷使用