?17-β-羥基類固醇脫氫酶14(HSD17b14)重組蛋白說明書
Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14)?
[PROPERTIES]
Residues: Met1~Ser270
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q9BPX1
Host: E. coli
Subcellular Location: Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.8
Predicted Molecular Mass: 32.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[USAGE]
Reconstitute in sterile PBS, pH7.2-pH7.4
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC?for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition
[ SEQUENCES ]
?The sequence of the target protein is listed below.?
MATGTRYAGK VVVVTGGGRG IGAGIVRAFV NSGARVVICD KDESGGRALE QELPGAVFILCDVTQEDDVK TLVSETIRRF GRLDCVVNNA GHHPPPQRPE ETSAQGFRQL LELNLLGTYTLTKLALPYLR KSQGNVINIS SLVGAIGQAQ AVPYVATKGA VTAMTKALAL DESPYGVRVNCISPGNIWTP LWEELAALMP DPRATIREGM LAQPLGRMGQ PAEVGAAAVF LASEANFCTGIELLVTGGAE LGYGCKASRS TPVDAPDIPS
本產品僅供科研使用。不能用于人和動物治療等其它臨床診斷使用