新品推薦EpiCypher——重磅推出H3K36me2抗體 貨號13-0056
H3K36me2 是一種與致癌轉錄程序相關的臨床相關染色質標記，尤其在由組蛋白甲基轉移酶 NSD2 失調的癌癥中具有重要意義。為了深入探索 H3K36me2 這一關鍵表觀遺傳標記，EpiCypher新推出了H3K36me2 抗體，該抗體經過多重嚴格驗證，專為精準的染色質分析而設計，是癌癥機制研究與表觀藥物發現領域的理想工具。
01 特異性高
該抗體在 CUT&Tag 實驗中符合 EpiCypher 的 SNAP-Certified™ 標準，在使用 SNAP-CUTANA™ 添加對照（染色質分析中定量特異性評估的金標準）檢測時，與相關組蛋白翻譯后修飾的交叉反應性低于 20%。
02 經過敲除驗證
在 NSD2 敲除后，該抗體在 CUT&Tag 中的富集信號消失，證實所觀察到的信號反映的是依賴于 NSD2 的 H3K36me2 沉積。
03 性能卓越
與其他市售的 H3K36me2 抗體相比，該抗體在 CUT&RUN 實驗中能提供更強、更具生物學準確性的信號。
04 批次間一致性高
每批產品均經過嚴格質控，包括在基因組定位分析中進行直接測試，以確保性能一致性。
05經研究文獻驗證
近期發表于《Nature》雜志的一項研究使用了該抗體來表征一種新型 NSD2 抑制劑的作用機制，助力揭示 NSD2 抑制的表觀基因組效應，證明了其在研究 NSD2 生物學功能及評估表觀遺傳療法中的應用價值 (Jeong et al. Nature (2026) PMID: 40770093)。
圖1. SNAP specificity analysis in CUT&Tag. CUT&Tag was performed as described above. CUT&Tag sequencing reads were aligned to the unique DNA barcodes corresponding to each nucleosome in the K-MetStat panel (x-axis). Data are expressed as a percent relative to on-target recovery(H3K36me2 set to 100%). Dotted line represents the 20% cross reactivity threshold for SNAP-certification.
圖2. CUT&Tag genome-wide enrichment. CUT&Tag was performed as described above. Sequence reads were aligned to 23,235 annotated genes and heatmaps were generated across gene bodies from TSS to TTS extending 2kbp upstream from the TSS and 2kbp downstream from the TTS. Signal enrichment was sorted from highest to lowest (top to bottom) relative to the H3K36me2 sample (all gene rows aligned). High, medium, and low intensity are shown in red, yellow, and blue, respectively. H3K4me3 positive control and H3K36me2 antibodies produced the expected enrichment patterns, which were higher than those observed with the IgG negative control.
圖3. H3K36me2 CUT&Tag representative browser tracks. CUT&Tag was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). H3K36me2 antibody tracks display the characteristic enrichment known to be consistent with the function of this PTM.
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貨號 |
產品名稱 |
規格 |
保存溫度 |
應用 |
|
13-0056 |
H3K36me2 CUTANA™ CUT&RUN and CUT&Tag Antibody |
100ug |
Stable for 1 year at 4°C from date of receipt |
CUT&Tag: 0.5 µg per reaction CUT&RUN: 0.5 µg per reaction |
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