?前列腺蛋白類脂肪酶B(LIPB)重組蛋白說明書
Recombinant Lipophilin B, Prostatein Like (LIPB)?
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Glu22~Val90
Tags: Two N-terminal Tags, His-tag and GST-tag
Tissue Specificity: Skeletal Muscle, Thymus. Subcellular Location: Secreted. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM
EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.6
Predicted Molecular Mass: 37.6kDa
Accurate Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are asfollows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
?2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of0.1-1.0
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
本產品僅供科研使用。不能用于人和動物治療等其它臨床診斷使用