?腺苷高半胱氨酸水解酶(AHCY)重組蛋白說(shuō)明書(shū)
Recombinant Adenosylhomocysteinase (AHCY)??
[DESCRIPTION]
Residues: Ser2~Tyr432
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P23526
Host: E. coli
Subcellular Location: Cytoplasm. Melanosome.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 51.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]?
Reconstitute in sterile PBS, pH7.2-pH7.4.
[STORAGE AND STABILITY]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
?
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37oC?for 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.
[SEQUENCES]
The sequence of the target protein is listed below.?
SDKLPYKVA DIGLAAWGRK ALDIAENEMP GLMRMRERYS ASKPLKGARI AGCLHMTVETAVLIETLVTL GAEVQWSSCN IFSTQDHAAA AIAKAGIPVY AWKGETDEEY LWCIEQTLYFKDGPLNMILD DGGDLTNLIH TKYPQLLPGI RGISEETTTG VHNLYKMMAN GILKVPAINVNDSVTKSKFD NLYGCRESLI DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ ALRGFGARVIITEIDPINAL QAAMEGYEVT TMDEACQEGN IFVTTTGCID IILGRHFEQM KDDAIVCNIGHFDVEIDVKW LNENAVEKVN IKPQVDRYRL KNGRRIILLA EGRLVNLGCA MGHPSFVMSNSFTNQVMAQI ELWTHPDKYP VGVHFLPKKL DEAVAEAHLG KLNVKLTKLT EKQAQYLGMSCDGPFKPDHY RY
本產(chǎn)品僅供科研使用。不能用于人和動(dòng)物治療等其它臨床診斷使用