?低密度脂蛋白受體相關蛋白關聯蛋白1(LRPAP1)重組蛋白說明書
Recombinant Low Density Lipoprotein Receptor Related Protein Associated Protein 1 (LRPAP1)??
[ PROPERTIES ]
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Residues: Glu90-Leu342 with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Endoplasmic reticulum.
Cytoplasm. Cell surface.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 31.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
?Store at 2-8oC for one month.
?Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
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[ SEQUENCES ]?
The target protein is fused with N-terminal His-Tag, its sequence is listed below.?
MGHHHHHHSGSEF-E LAWKKLKLDG LDEDGEKEAR LIRNLNVILA KYGLDGKKDARQVTSNSLSG TQEDGLDDPR LEKLWHKAKT SGKFSGEELD KLWREFLHHK EKVHEYNVLLETLSRTEEIH ENVISPSDLS DIKGSVLHSR HTELKEKLRS INQGLDRLRR VSHQGYSTEAEFEEPRVIDL WDLAQSANLT DKELEAFREE LKHFEAKIEK HNHYQKQLEI AHEKLRHAESVGDGERVSRS REKHALLEGR TKELGYTVKK HL
本產品僅供科研使用。不能用于人和動物治療等其它臨床診斷使用