?腫瘤壞死因子受體超家族成員4(TNFRSF4)重組蛋白說明書
Recombinant Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4)
[ PROPERTIES ]
Residues: Lys29~Gly210
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.5
Predicted Molecular Mass: 21.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in? ddH2O
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
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[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-KH TYPSGHKCCR ECQPGHGMVS RCDHTRDTLC HPCETGFYNEAV NY D TCKQC TQ CNHRSGSE LKQN CTPTQD TV C RCRPG TQ PR QDSGYK LGVDCVPCPPGH FSPGNNQACK PWTNCTLSGK QTRHPASDSL DAVCEDRSLL ATLLWETQRPTFRPTTVQST TVWPRTSELP SPPTLVTPEG
[ REFERENCES ]
1. Calderhead D.M., et al. (1993) J. Immunol. 151:5261-5271.
2. Birkeland M.L., et al. (1995) Eur. J. Immunol. 25:926-930.
3. Pippig S.D., et al. (1999) J. Immunol. 163:6520-6529.
4. Kopf M., et al. (1999) Immunity 11:699-708.
本產品僅供科研使用。不能用于人和動物治療等其它臨床診斷使用