干擾素α10(IFNa10)重組蛋白說(shuō)明書(shū)-分析方法-資訊-生物在線

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干擾素α10(IFNa10)重組蛋白說(shuō)明書(shū)

作者:上海滬震實(shí)業(yè)有限公司 2023-04-24T00:00 (訪問(wèn)量:802)

干擾素α10(IFNa10)重組蛋白說(shuō)明書(shū)

Recombinant Interferon Alpha 10 (IFNa10)?

[ DESCRIPTION ]

Protein Names: Interferon Alpha 10

Synonyms: IFNa10

Species:? Human

Size: 100μg

Source:? Escherichia coli-derived

Subcellular? Location: Secreted.

[ PROPERTIE]

Residues:? Cys24~Asp189 with N-terminal His-Tag.

?Grade & Purity:>95%, 23kDa as determined bySDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose.

Endotoxin? Level: <1.0 EU per 1μg (determined bythe LAL method).

Applications:? SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted? Molecular Mass: 20.9kDa

Predicted isoelectric point: 6.1

[USAGE]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

?

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oCfor 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

?

[ SEQUENCES]?

The target protein is fused with N-terminal His-Tag, its sequence is listed below.?

MGHHHHHHSGSEF-CDLPQTH SLGNRRALIL LGQMGRISPF SCLKDRHDFR IPQEEFDGNQFQKAQAISVL HEMIQQTFNL FSTEDSSAAW EQSLLEKFST ELYQQLNDLE ACVIQEVGVEETPLMNEDSI LAVRKYFQRI TLYLIERKYS PCAWEVVRAE IMRSLSFSTN LQKRLRRKD

2022120407172703277

本產(chǎn)品僅供科研使用。不能用于人和動(dòng)物治療等其它臨床診斷使用

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