我們的HEK-BlueTM細胞因子報告細胞作為一個不斷拓展的工程化細胞系系列,旨在提供一種簡單快速且可靠的方法來監測關鍵細胞因子誘導的信號通路激活。每個批次都經過功能測試和驗證,以確保性能的一致性。每個HEK-BlueTM細胞因子報告細胞系都有一個分泌型胚胎堿性磷酸酶SEAP(secreted embryonic alkaline phosphatase)報告基因,該基因受所研究細胞因子誘導的轉錄因子調控。使用Quanti-BlueTM Solution可以輕松評估SEAP活性。這些細胞能靈敏監測具有生物活性的細胞因子,是篩選具有激動劑或拮抗劑活性的化合物(如中和抗體)的理想選擇。如需購買Invivogen公司產品,請聯系我們的代理商欣博盛生物。
主要特點
• HEK293衍生的工程化細胞系
• 易于比色讀數和快速定量
• 對目標細胞因子具有高靈敏度
• 經細胞因子特異性測試
• 經認證不含支原體
• 至少可穩定傳代 20 次
應 用
• 開發治療方案
• 藥物篩選
• 釋放試驗

?? 每種產品的所有數據均可在我們的網站上找到。
| 產品 | 檢測到的細胞因子/通路 | 報告基因 | 貨號 |
| 干擾素報告細胞 | |||
| HEK-Blue™ IFN-α/β | Human&mouse type I IFNs/STAT1-STAT2-IRF9 (ISGF3) | SEAP | hkb-ifnabv2 |
| HEK-Blue™ IFN-γ | Human type II IFN/STAT1 | SEAP | hkb-ifng |
| HEK-Blue™ IFN-λ | Human&mouse type III IFNs (IL-28a, IL-28b, IL-29)/STAT1-STAT2-IRF9(ISGF3) | SEAP | hkb-ifnlv2 |
| 白介素報告細胞 | |||
| HEK-Blue™ IL-1β | Human&mouse IL-1α/β, knock-out of TNFR1/NF-κB | SEAP | hkb-il1bv2 |
| HEK-Blue™ IL-1R | Human&mouse IL-1α/β/NF-κB | SEAP | hkb-il1r |
| HEK-Blue™ IL-2/IL-15 | Human&mouse IL-2, human IL-15/STAT5 | SEAP | hkb-il2 |
| HEK-Blue™ CD122/CD132 | Human&mouse IL-2, human IL-15/STAT5 | SEAP | hkb-il2bg |
| HEK-Blue™ IL-3 | Human IL-3/STAT5 | SEAP | hkb-il3 |
| HEK-Blue™ IL-4/IL-13 | Human IL-4 and human&mouse IL-13/STAT6 | SEAP | hkb-il413 |
| HEK-Blue™ IL-5 | Human&mouse IL-5/STAT5 | SEAP | hkb-il5 |
| HEK-Blue™ IL-6 | Human IL-6/STAT3 | SEAP | hkb-hil6 |
| HEK-Blue™ IL-7 | Human&mouse IL-7/STAT5 | SEAP | hkb-il7 |
| HEK-Blue™ IL-9 | Human&mouse IL-9/STAT5 | SEAP | hkb-il9 |
| HEK-Blue™ IL-10 | Human&mouse IL-10/STAT3 | SEAP | hkb-il10v2 |
| HEK-Blue™ IL-11 | Human&mouse IL-11/STAT3 | SEAP | hkb-hil11rv2 |
| HEK-Blue™ IL-12 | Human&mouse IL-12/STAT4 | SEAP | hkb-il12 |
| HEK-Blue-GFP™ IL-12 | Human&mouse IL-12/STAT4 | SEAP/GFP | hkbg-il12 |
| HEK-Blue™ IL-17 | Human IL-17A/F and human&mouse IL-17E/NF-κB | SEAP | hkb-il17 |
| HEK-Blue™ IL-17C | Human&mouse IL-17C/E and human IL-17A/NF-κB | SEAP | hkb-il17c |
| HEK-Blue™ IL-18 | Human&mouse IL-18/NF-κB | SEAP | hkb-hmil18 |
| HEK-Blue™ IL-19/IL-20 | Human&mouse IL-19, IL-20&IL-24/STAT3 | SEAP | hkb-il1920 |
| HEK-Blue™ IL-20 | Human&mouse IL-20 IL-22&IL-24/STAT3 | SEAP | hkb-il20 |
| HEK-Blue™ IL-21 | Human&mouse IL-21/STAT3 | SEAP | hkb-il21 |
| HEK-Blue™ IL-22 | Human&mouse IL-22/STAT3 | SEAP | hkb-il22 |
| HEK-Blue™ IL-23 | Human&mouse IL-23/STAT3 | SEAP | hkb-il23 |
| HEK-Blue™ IL-31 | Human IL-31/STAT5 | SEAP | hkb-il31 |
| HEK-Blue™ IL-33 | Human&mouse IL-33/NF-κB | SEAP | hkb-hil33 |
| HEK-Blue™ IL-36 | Human IL-36/NF-κB | SEAP | hkb-hil36r |
| 生長因子、集落刺激因子和類激素報告細胞 | |||
| HEK-Blue™ EPO | Human&mouse EPO/STAT5 | SEAP | hkb-epo |
| HEK-Blue™ G-CSF | Human&mouse GM-CSF/STAT3 | SEAP | hkb-gcsf |
| HEK-Blue™ GM-CSF | Human GM-CSF/STAT5 | SEAP | hkb-hgmcsfr |
| HEK-Blue™ GLP-1 | Human GLP1/CREB | SEAP | hkb-glp1 |
| HEK-Blue™ TGF-β | Human&mouse TGF-β/Smad | SEAP | hkb-tgfb |
| HEK-Blue™ TPO | Human&mouse TPO/STAT5 | SEAP | hkb-tpo |
| HEK-Blue™ TSLP | Human TSLP/STAT5 | SEAP | hkb-tslp |
| 腫瘤壞死因子相關報告細胞 | |||
| HEK-Blue™ BAFF-R | Human&mouse BAFF/NF-κB | SEAP | hkb-baffr |
| HEK-Blue™ BCMA | Human&mouse APRIL, human&mouse BAFF/NF-κB | SEAP | hkb-bcma |
| HEK-Blue™ CD40L | Human&mouse CD40L/NF-κB | SEAP | hkb-cd40 |
| HEK-Blue™ RANKL | Human&mouse RANKL/NF-κB | SEAP | hkb-ranklv2 |
| HEK-Blue™ TACI | Human&mouse APRIL, human&mouse BAFF/NF-κB | SEAP | hkb-taci |
| HEK-Blue™ TNF-α | Human&mouse TNF-α/NF-κB | SEAP | hkb-tnfdmyd |
| HEK-Blue™ TL1A | Human&mouse TL1A/NF-κB | SEAP | hkd-tl1a |
| 特色產品 | HEK-BLUE™ TSLP CELLS |
|
Signaling pathway in HEK-BlueTM TSLP cells |
(A) HEK-Blue™ TSLP cells were stimulated with increasing concentrations of human (h) or mouse (m) TSLP. (B) Increasing concentrations of Anti-hTSLP-hIgG2 (0.3 ng/ml - 30μg/ml) were incubated with hTSLP (1ng/ml) for 1 h before the addition of HEK-Blue™ TSLP cells. (C) HEK-Blue™ TSLP cells were stimulated with various human and mouse recombinant cytokines:10ng/ml of hTSLP, mTSLP, hIFN-γ, hTNF-α, hIL-1β, hIL-2, hIL-7, hIL-9, hIL-15, and 1000 U/ml hIFN-α. After overnight incubation, SEAP activity was assessed using QUANTI?Blue™ Solution. The optical density (OD) at 650 nm is shown as mean ± SEM. |
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細胞因子優惠 | ![]() |
Additional cell vial | |
| 每購買一只細胞因子報告細胞系, 即可免費獲贈一瓶相應的細胞因子。 | 購買相同細胞時,第二支起可享優惠。 詳情請參閱網站上的條款。 |
|||
| 詳情請參閱網站上的條款。 | invivogen.com/cytokine-reporter-cells |
|
相關產品 |
貨號 |
|
QUANTI-Blue™ Solution |
rep-qbs |
|
Normocin® |
ant-nr-1 |
|
Blasticidin |
ant-bl-05 |
|
Zeocin® |
ant-zn-05 |
|
Puromycin |
ant-pr-1 |
|
HEK-Blue™ Selection |
hb-sel |
更多詳情請聯系 InvivoGen 中國授權代理-欣博盛生物
全國服務熱線: 4006-800-892 郵箱: market@neobioscience.com
深圳: 0755-26755892 北京: 010-88594029 上海: 021-34613729
廣州:020-87615159 香港: 852-69410778
代理品牌網站: www.nbs-bio.com
欣博盛品牌網站: www.neobioscience.net




