eBioscience 最新推出了應用于紫光激發的超高性能新型熒光染料-Super Bright系列。該系列熒光染料均已最大發射波長命名。這些染料經過優化尤其適用于流式染色。與傳統類似染料相比，Super Bright 染料的非特異性結合更少。Super Bright系列染料兼容其他eBioscience的染料、緩沖液、固定劑和UltraComp eBeads 補償微球。
Super Bright 600是串聯染料，由Super Bright 436和受體染料串聯而成。
? 激發光：405nm
? 發射光：600nm
? 可替代染料：eVolve 605， Violet 605 (BV605
? 檢測濾片：610/20 bandpass
檔案詳細資料：
1. Super Bright 436 vs Brilliant Violet 605：亮度與BV421相當，尤其適合低豐度表達的抗原。
| Super Bright 600 antibody conjugates offer similar brightness to Brilliant Violet 605-conjugated antibodies.(A) Human peripheral blood cells were stained with Anti-CD3 (clone OKT3)conjugated to Super Bright 600 (red histogram) or Brilliant Violet 605 (grey histogram), using the same concentration of antibody. (B) Mouse splenocytes were stained with Anti-CD8a (clone 53-6.7) conjugated to Super Bright 600 (red histogram) or Brilliant Violet? 605 (grey histogram), using the same concentration of antibody. |  |
2. Super Bright 436 vs eVolve 605：亮度比eVolve 605要高。
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Human peripheral blood lymphocytes were stained with Anti-CD3 (clone OKT3)conjugated to Super Bright 600 (red histogram) or eVolve 605 (blue histogram), using the same concentration of antibody. |
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(A) Mouse splenocytes were stained with Anti-CD4 (clone RM4-5) Super Bright 600and were either analyzed immediately (red histogram), left in the dark overnight (blue histogram), or left exposed to light overnight (green histogram). (B) Human peripheral blood cells were stained with Anti-CD8a (clone RPA-T8) Super Bright 600 and were either analyzed immediately (red histogram), left in the dark overnight (blue histogram), or left exposed to light overnight (green histogram). |
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Stability studies indicate that Super Bright 600 exhibits a minimal loss of fluorescence when cells are exposed to various fixatives Mouse lymphocytes were stained with Anti-CD45R/B220 (clone RA3-6B2) Super Bright 600 and:
(A) were left unfixed (red histogram), or fixed in IC Fixation buffer for 30 minutes (blue histogram), 24 hours (orange histogram), or three days (green histogram), followed by a wash in Permeabilization buffer.
(B) were left unfixed (red histogram), or fixed in Foxp3/Transcription Factor/Permeabilization buffer for 30 minutes (blue histogram), 24 hours (orange histogram), or three days (green histogram), followed by a wash in Permeabilization buffer.
(C): were left unfixed (red histogram), or fixed in IC Fixation buffer followed by 90% methanol for 30 minutes (blue histogram), 24 hours (orange histogram), or three days (green histogram), followed by a Flow Cytometry Staining Buffer wash.
5. 多色分析示例- Super Bright 600.
Normal human peripheral blood cells were aliquoted in the presence of Super Bright Staining Buffer (Cat. No. SB-4400), then surface stained with the indicated reagents. Viable cells, as determined by staining with Fixable Viability Dye eFluor? 780, were used for analysis to discriminate various T cell subsets.