蛋白酶是一類天然存在的調節酶，用于切割多肽鏈氨基酸之間的肽鍵。在蛋白表達和分離過程中，內源性細胞蛋白酶可以快速消化細胞蛋白，致使蛋白質樣品質量和數量大幅減少，這是蛋白表征和分析所需的。BioVision引入了一個新的常規產品線，也是特別的蛋白酶抑制劑和混合物，可以防止體外蛋白水解。這些蛋白酶抑制劑和混合物簡單易用，可用于抑制廣泛的蛋白酶。抑制劑混合物套裝包含最佳濃度的抑制劑的不同組合，為研究者提供多種選擇，包括不含EDTA的形式。
蛋白質磷酸化是可逆的蛋白翻譯后修飾（PTM）中最常見和最重要的形式，所有蛋白質在任何給定的時間高達30%可被磷酸化。大范圍的生物過程受磷酸化與去磷酸化蛋白的控制。為了了解蛋白質的精確磷酸化狀態，從組織和細胞裂解液中提取的磷酸化蛋白保持蛋白質的磷酸化狀態是非常重要的。BioVision有五種不同的EZBlock?磷酸酶抑制劑混合物和兩種新的通用的蛋白酶和磷酸酶抑制劑混合物，抑制蛋白質提取和純化過程中蛋白酶和磷酸酶活性。
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Product Name
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Cat #
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Target Proteases/Phosphatases
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EZBlock? Protease Inhibitor Cocktail
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K271-500
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Aspartic, Cysteine, Serine proteases,
and Esterases
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EZBlock? Protease Inhibitor Cocktail,
EDTA-Free |
K272-1, 5, set
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Aspartic, Cysteine, Serine, Trypsin-like
proteases, Aminopeptidases, and Esterases
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EZBlock? Protease Inhibitor Cocktail II
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K277-set
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Cysteine, Serine, Trypsin-like proteases, Aminopeptidases, Metalloproteases
and Esterases
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EZBlock? Free Protease Inhibitor
Cocktail II, Animal-Free |
K394-1,set
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Cysteine, Serine, Trypsin-like proteases, Aminopeptidases, Metalloproteases and
Esterases
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EZBlock? Protease Inhibitor Cocktail III
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K278-set
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Aspartic, Cysteine, Serine, Trypsin-like
proteases, Aminopeptidases,
Metalloproteases |
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EZBlock? Protease Inhibitor Cocktail IV
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K279-1,set
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Aspartic, Cysteine, Serine proteases,
and Metalloproteases
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EZBlock? Free Protease Inhibitor
Cocktail V, EDTA-Free |
K290-1,set
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Cysteine and Serine proteases
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EZBlock? Use Protease Inhibitor
Cocktail VI, General Use |
K291-1,set
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Cysteine, Serine proteases, Aminopeptidases
and Metalloproteases
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EZBlock? Protease Inhibitor Cocktail VII
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K292-1,set
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Aspartic, Cysteine , Serine proteases,
Aminopeptidases and Metalloproteases
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EZBlock? Protease Inhibitor Cocktail VIII
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K293-1,set
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Aspartic, Cysteine , Serine proteases,
Aminopeptidases and thermolysin-like
proteases
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EZBlock? Free Protease Inhibitor
Cocktail VIII, DMSO-Free |
K395-1,set
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Aspartic, Cysteine , Serine proteases,
Aminopeptidases and thermolysin-like
proteases
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EZBlock? Protease Inhibitor
Cocktail IX |
K294-1,set
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Cysteine proteases, including Calpains,
Cathepsins and Papain.
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EZBlock? Free Protease Inhibitor Cocktail X, EDTA-Free
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K297-1,set
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Cysteine, Serine and Aminopeptidases
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EZBlock? Free, Animal-Free Protease Inhibitor Cocktail XII, EDTA Free, Animal-Free
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K221-1,set
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Cysteine and Serine proteases
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EZBlock? Free, Animal-Free Protease Inhibitor Cocktail XIII, EDTA Free, Animal-Free
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K222-1,set
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Aspartic , Cysteine and Serine proteases, Aminopeptidases, Acid proteases
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EZBlock? Protease Inhibitor Cocktail XIV
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K223-1,set
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Cysteine proteases including Cathepsins
and Papain
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EZBlock? Protease Inhibitor Cocktail XV
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K393-1,set
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Cysteine proteases including Cathepsins
and Papain
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EZBlock? Phosphatase Inhibitor Cocktail I
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K273-1,set
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Acid phosphatases, Alkaline phosphatases,
Ser/Thr Phosphatases, Protein Tyrosine
Phosphatases
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EZBlock? Phosphatase Inhibitor Cocktail II
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K275-1,set
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Acid phosphatases, Alkaline phosphatases,
Ser/Thr Phosphatases, Protein Tyrosine
Phosphatases
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EZBlock? Phosphatase Inhibitor Cocktail III
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K276-1,set
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Alkaline phosphatases, Ser/Thr
Phosphatases (PP1 and PP2A)
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EZBlock? Phosphatase Inhibitor Cocktail IV
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K282-1,set
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Acid phosphatases, Alkaline phosphatases,
Ser/Thr Phosphatases, Protein Tyrosine
Phosphatases
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EZBlock? Phosphatase Inhibitor Cocktail XI
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K298-1,set
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Acid phosphatases, Alkaline phosphatases,
Protein Tyrosine Phosphatases
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EZBlock? Universal protease &
Phosphatase Inhibitor Cocktail |
K283-1,set
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Cysteine, Serine, Trypsin-like proteases, Aminopeptidases, Metalloproteases and
Esterases, Acid phosphatases, Alkaline
phosphatases, Ser/Thr Phosphatases,
Protein Tyrosine Phosphatases
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EZBlock? Universal protease &
Phosphatase Inhibitor Cocktail, EDTA-Free |
K284-1,set
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Cysteine, Serine, Trypsin-like proteases, Aminopeptidases, Metalloproteases and
Esterases, Acid phosphatases, Alkaline
phosphatases, Ser/Thr Phosphatases,
Protein Tyrosine Phosphatases
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