促銷時間：2016年3月15-2016年6月14日

促銷內容：原價是4122 促銷價格是3500

Sciencell公司產品由上海中喬新舟生物科技有限公司優質供應

美國ScienCell研究實驗室（www.sciencellonline.com）成立于1999年，公司總部位于美國加州的圣地亞哥。主要致力
于實驗室科研用原代細胞、原代細胞專用培養基、原代細胞無血清培養基、干細胞、干細胞培養基、干細胞無血清培養
基的研究和開發，在全球擁有眾多客戶。在國內銷售15年來，很多老師應用其產品發表了高質量的SCI文章，且有著極高
的文獻引用率。憑借著嚴格的質控和優秀的產品品質，深受廣大科研工作者的信賴。

ScienCell研究實驗室生產的原代細胞、原代細胞專用培養基都經過了嚴格的質量控制，細胞純度可達98％。其中包括21
種人體正常細胞系統，90多種不同細胞類型。大多數細胞在全球唯有ScienCell實驗室能夠成功分離，產品質量過硬。
確保了實驗結果的重復性和連貫性。

請登錄Sciencell公司官方網站（www.zqxzbio.com 或www.sciencellonline.com ）以確保購買正規公司產品

人臍靜脈內皮細胞說明：
貨號：8000


血管內皮細胞對維持血管動態平衡起著重要作用。它們合成、分泌凝血和纖溶系統的激活因子和抑制因子、影響血
小板粘附和聚集的調節因子。內皮細胞還釋放控制細胞增殖和調節血管壁緊張度的分子。這些過程都可以用培養的
細胞進行體外研究，人臍靜脈和動脈內皮細胞是這類研究中最常用的細胞類型。除了普通的內皮細胞特征例如
“鵝卵石狀”，VIII因子陽性著色，能攝取乙酰基化低密度脂蛋白，人臍血管內皮細CD31染色陽性。用IL-1或TNF-α
預先處理過的細胞也能選擇性地表達E-selectin和VCAM。 人臍靜脈內皮細胞(HUVEC)提取于人臍帶靜脈組織，
原代凍存。每管含有細胞數>5×105 cells/ml，此細胞通過vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗證，
經測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。細胞可以達到15倍增。

推薦培養基:(ECM, Cat. No. 1001)

The vascular endothelial cells contribute to the maintenance of vascular homeostasis. They synthesize and
secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition
to mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release
molecules that control cell proliferation and modulate vessel wall tone. Many of the endothelial processes
can be studied in vitro using cultured cells, and human umbilical vein endothelial cells (HUVEC) are the
most commonly used cell type for such studies. Except for the common endothelial cell features such as
"cobblestone" morphology, positive staining for Factors VIII and the ability to take up acetylated low-density
lipoprotein [1], HUVEC stein positively with CD-31 [2]. Cells pretreated with IL-1 or TNF-alpha also selectively
express E-selectin [3] and VCAM [4].
HUVEC from ScienCell Research Laboratories are isolated from human umbilical vein. HUVEC are
cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HUVEC
are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM)
and by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and
fungi. HUVEC are guaranteed to further expand for 15 population doublings at the conditions provided by
ScienCell Research Laboratories.

Reference
1. Morgan, D. M. L. (1996) Isolation and culture of human umbilical vein endothelial cells. In "Human Cell
Culture Protocols" (Gareth E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109.
2. Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin
gene superfamily. Science 247: 1219-1222.
3. Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils
related to complement regulatory proteins and lectins. Science 243:1`160-1165.
4. Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial
protein that binds to lymphocytes. Cell 59:1203-1211.

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