eBioscience 最新推出了應(yīng)用于紫光激發(fā)的超高性能新型熒光染料-Super Bright系列。該系列熒光染料均已最大發(fā)射波長命名。這些染料經(jīng)過優(yōu)化尤其適用于流式染色。與傳統(tǒng)類似染料相比，Super Bright 染料的非特異性結(jié)合更少。Super Bright系列染料兼容其他eBioscience的染料、緩沖液、固定劑和UltraComp eBeads 補(bǔ)償微球。
激發(fā)光：405nm
發(fā)射光：436nm
可替代染料：eFluor 450, Brilliant? Violet 421 (BV421)
檢測濾片：450/50 bandpass
檔案詳細(xì)資料：
1. Super Bright 436 vs Brilliant Violet 421：；亮度與BV421相當(dāng)甚至更好。
(A)\Human peripheral blood cells were stained with Anti-CD19 (clone HIB19)conjugated to Super Bright 436 (purple histogram) or to Brilliant Violet 421 (blue histogram) using the manufacturer’s recommended volume per test.
(B) and (C)\Mouse bone marrow cells were stained with Anti-CD117 (clone 2B8) APC and Anti-Ly-6A/E (clone D7) conjugated to either Super Bright 436 (B) or to Brilliant Violet 421
(C) at the same antibody concentration.
2. Super Bright 436 比eFluor 450亮度高
Mouse bone marrow cells were stained with Anti-CD117(clone 2B8) conjugated to (A) Super Bright 436, or (B) eFluor 450.
3. Super Bright 436光穩(wěn)定性
Human peripheral blood cells were stained with Anti-CD19 (clone HIB19) conjugated to (A)Super Bright 436 or (B) to Brilliant Violet 421, andeither left in the dark (red histogram), or exposed to ambient light for four hours (orange histogram) or overnight (blue histogram).
4. Super Bright 436 固定后的穩(wěn)定性
Stability studies indicate that Super Bright 436 exhibits a minimal loss of fluorescence when cells are exposed to various fixatives. Human peripheral blood cells were stained with Anti-CD27 (clone O323) Super Bright 436 and:
(A) were left unfixed (red histogram), or fixed in IC Fixation buffer for 30 minutes (blue histogram), overnight (orange histogram), or three days (green histogram), followed by a wash in Permeabilization buffer.
(B) were left unfixed (red histogram), or fixed in Foxp3/Transcription Factor buffer for 30 minutes (blue histogram), overnight (orange histogram), or three days (green histogram), followed by a wash in Permeabilization buffer.
(C) were left unfixed (red histogram), or fixed in IC Fixation buffer followed by 90% methanol for 30 minutes (blue histogram), overnight (orange histogram), or three days (green histogram), followed by a wash in Flow Cyometry Staining Buffer.
Mouse splenocytes were stained with a three-color panel comprised of Anti-CD45R/B220 (clone RA3-6B2) Super Bright 436, Anti-CD8a (clone 53-6.7) Super Bright 600, and Anti-CD4 (clone GK1.5) Super Bright 645. Compensation was set using (A) UltraComp eBeads microspheres (top row) or (B) with cells (bottom row). Compensation values with beads were similar to single color stained cells
(not shown).