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Biotinylated HER2 Aids in the Quantification of Antibodies in Serum
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Two recent publications have highlighted the use of biotinylated HER2 protein in quantification of
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In a newly published US patent titled "Antibody‐Drug Conjugate Having Improved Stability and Use thereof" (US patent 20160136300), Kim et al. used ACROBiosystems'biotinylated HER2 (HE2‐H822R) as a detection antibody in a sandwich ELISA to measure anti‐HER2 antobodies in the serum.
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Meanwhile, Chen et al. from Agilent Technology Inc. used biotinylated HER2-ECD,Catalog #HE2‐H822R, (produced by in‐house biotinylation of ACROBiosystems' HER2‐ECD protein HE2‐H5225) for affinity purification of anti‐HER2 ADC from rat serum. The purified ADC is further analyzed by LC/MS.
This work is published on an Agilent application note titled "Determination of Drug‐to‐Antibody Ratio for Antibody‐Drug Conjugates Purified from Serum"
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ACROBiosystems' Biotinylated HER2 (HE2‐H822R) Bioactivity Data
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Immobilized trastuzumab at 3 ug/mL (100ul/well) can bind Biotinylated Human Her2,Ultra Sensitivity (Catalog #HE2‐H822R). The EC50 of Biotinylated Human Her2, Superior Sensitivity is 0.09‐1 ng/ml.
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